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In vitro cytotoxicity studies of industrial Eucalyptus kraft lignins on mouse hepatoma, melanoma and Chinese hamster ovary cells.

Abstract
Kraft lignin is a polyphenolic compound generated as a by-product from the kraft pulping process in large quantities annually worldwide. In addition to its commercial availability, its structural features make it worth to be considered in the pharmaceutical area. The present study was carried out to evaluate in vitro antioxidant and cytotoxic properties of kraft lignin on mouse hepatoma MH-22A, melanoma B16 (tumor cells) and Chinese hamster ovary (CHO, non-cancerous) cells. Moreover, several analytical techniques were used in order to elucidate the chemical structure of isolated industrial lignins (FT-IR, GPC, Py-GC-MS, 2D HSQC NMR). Results revealed high phenolic content in their composition, high-condensed structure and high phenolic hydroxyls group content. DPPH and ABTS⁎+ radical scavenging assays demonstrated their strong antioxidant activity, which was higher than found for commercial antioxidant (BHT). Kraft lignins act cytotoxically inducing apoptosis- and necrosis-like processes on both on tumor and normal cells. However, the results evidenced that MH-22A cells showed greater sensitive behavior than B16 and non-cancerous CHO cells, which were more tolerant of kraft lignin.
AuthorsOihana Gordobil, Alona Oberemko, Gintautas Saulis, Vykintas Baublys, Jalel Labidi
JournalInternational journal of biological macromolecules (Int J Biol Macromol) Vol. 135 Pg. 353-361 (Aug 15 2019) ISSN: 1879-0003 [Electronic] Netherlands
PMID31125648 (Publication Type: Journal Article)
CopyrightCopyright © 2019 Elsevier B.V. All rights reserved.
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Phytochemicals
  • Kraft lignin
  • Lignin
Topics
  • Animals
  • Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
  • Antioxidants (chemistry, isolation & purification, pharmacology)
  • CHO Cells
  • Carcinoma, Hepatocellular
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • Chemical Phenomena
  • Cricetulus
  • Eucalyptus (chemistry)
  • Lignin (chemistry, isolation & purification, pharmacology)
  • Liquid-Liquid Extraction
  • Melanoma
  • Mice
  • Phytochemicals (chemistry)
  • Spectrum Analysis

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