Overexpression of the long noncoding RNA TRHDE-AS1 inhibits the progression of lung cancer via the miRNA-103/KLF4 axis.

Abstract

AIM:

The aim of this study was to explore the role and molecular basis of the long noncoding RNA (lncRNA) TRHDE-AS1 in lung cancer.

METHODS:

We used real-time polymerase chain reaction to analyze the messenger RNA expression levels of TRHDE-AS1, miR-103, and KLF4. The cell viability, proliferation, and invasion rates were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cell Counting Kit-8, and Transwell assays to elucidate the role of TRHDE-AS1.

RESULTS:

Our results demonstrated that the lncRNA TRHDE-AS1 is mainly located in the cytoplasm and that the cell proliferation and invasion were suppressed in the group of overexpressed TRHDE-AS1. We also showed that miR-103 could directly bind to TRHDE-AS1 and provided evidence of the oncogenic function of miR-103. Besides, we proved that miR-103 exerted its function by adjusting the expression level of the tumor-suppressor gene KLF4, and the expression level was negatively associated with miR-103.

CONCLUSION:

In summary, we determined that the effects of TRHDE-AS1 on proliferation, invasion, and cell death could be rescued by the overexpression of miR-103. Our experiments demonstrate that the TRHDE-AS1/miR-103/KLF4 axis may provide new evidence for understanding the molecular basis of lung cancer.

• Authors: Bing Zhuan, Yuting Lu, Qian Chen, Xia Zhao, Ping Li, Qun Yuan, Zhao Yang
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 120 Issue 10 Pg. 17616-17624 (10 2019) ISSN: 1097-4644 [Electronic] United States
• PMID: 31119790 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Wiley Periodicals, Inc.

Chemical References

• KLF4 protein, human
• Klf4 protein, mouse
• Kruppel-Like Factor 4
• Kruppel-Like Transcription Factors
• MIRN103 microRNA, human
• MicroRNAs
• RNA, Long Noncoding

Topics

• A549 Cells
• Animals
• Apoptosis (genetics)
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Kruppel-Like Factor 4
• Kruppel-Like Transcription Factors (genetics)
• Lung Neoplasms (genetics, pathology)
• Male
• Mice
• MicroRNAs (genetics)
• Neoplasm Invasiveness (genetics, pathology)
• RNA, Long Noncoding (genetics)
• Signal Transduction (genetics)
• Xenograft Model Antitumor Assays