Comparison of quantitative acid-elution technique and flow cytometry for detecting fetomaternal hemorrhage.

Abstract

A study of the Kleihauer-Betke acid-elution technique for quantitating fetomaternal hemorrhage was performed to assess intra- and inter-technologist accuracy and precision as well as to delineate the statistically valid domain of the test as usually performed. The results were then compared to a parallel study quantitating fetomaternal hemorrhage by flow cytometry. Additionally, a statistical model for estimating efficacy of treatment with Rh immune globulin in the prevention of pregnancy-associated Rh(D) isoimmunization was developed. The results indicate that the acid-elution technique can be performed in a reproducible manner with acceptable accuracy and precision with whole blood fetomaternal hemorrhages 25 ml and higher if a background correction for false positive identification of fetal cells is included. Flow cytometric determination reveals significantly increased accuracy in comparison to the corresponding Kleihauer-Betke results.

• Authors: J P Corsetti, C Cox, J F Leary, M T Cox, N Blumberg, R A Doherty
• Journal: Annals of clinical and laboratory science (Ann Clin Lab Sci) 1987 May-Jun Vol. 17 Issue 3 Pg. 197-206 ISSN: 0091-7370 [Print] United States
• PMID: 3111343 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Topics

• Adult
• Erythrocytes (cytology)
• Female
• Fetal Blood (cytology)
• Fetomaternal Transfusion (diagnosis)
• Flow Cytometry
• Humans
• Hydrogen-Ion Concentration
• Immunoassay (methods)
• Models, Biological
• Predictive Value of Tests
• Pregnancy
• Rh Isoimmunization (prevention & control)