Abstract | BACKGROUND: OBJECTIVE: METHODS: Male Sprague Dawley rats (n = 48; 6 wk of age) were randomly assigned to a casein-based diet (C; n = 12), a casein-based diet supplemented with choline (C + Cho; 1.3%, wt:wt; n = 12), an egg protein-based diet (EP; n = 12), or a whole egg-based diet (WE; n = 12). At week 2, half of the rats in each of the 4 dietary groups were provided an FR (0 g folic acid/kg) diet and half continued on the folate-sufficient (FS; 0.2 g folic acid/kg) diet for an additional 6 wk. All diets contained 20% (wt:wt) total protein. Serum homocysteine was measured by HPLC and BHMT and CBS expression and activity were evaluated using real-time quantitative polymerase chain reaction, Western blot, and enzyme activity. A 2-factor ANOVA was used for statistical comparisons. RESULTS: Rats fed FR-C exhibited a 53% increase in circulating homocysteine concentrations compared with rats fed FS-C (P < 0.001). In contrast, serum homocysteine did not differ between rats fed FS-C and FR-EP (P = 0.078). Hepatic BHMT activity was increased by 45% and 40% by the EP (P < 0.001) and WE (P = 0.002) diets compared with the C diets, respectively. CONCLUSIONS:
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Authors | Cassondra J Saande, Samantha K Pritchard, Deanna M Worrall, Sarah E Snavely, Caitlyn A Nass, Joshua C Neuman, Rebecca A Luchtel, Sarah Dobiszewski, Joshua W Miller, Mario Vailati-Riboni, Juan J Loor, Kevin L Schalinske |
Journal | The Journal of nutrition
(J Nutr)
Vol. 149
Issue 8
Pg. 1369-1376
(08 01 2019)
ISSN: 1541-6100 [Electronic] United States |
PMID | 31111947
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © American Society for Nutrition 2019. |
Chemical References |
- Egg Proteins, Dietary
- RNA, Messenger
- Betaine-Homocysteine S-Methyltransferase
- Cysteine
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Topics |
- Animals
- Betaine-Homocysteine S-Methyltransferase
(genetics, metabolism)
- Body Weight
- Cysteine
(blood)
- Egg Proteins, Dietary
(administration & dosage, metabolism)
- Folic Acid Deficiency
(metabolism)
- Hyperhomocysteinemia
(prevention & control)
- Liver
(enzymology)
- Male
- RNA, Messenger
(genetics)
- Rats
- Rats, Sprague-Dawley
- Up-Regulation
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