The Pathological Significance and Prognostic Roles of Thrombospondin-1, and -2, and 4N1K-peptide in Bladder Cancer.

Abstract

BACKGROUND/AIM:

Thrombospondins (TSPs) play a role as inhibitors of angiogenesis under various pathological conditions. The aim of the study was to evaluate the pathological significance and prognostic role of the 4N1K-peptide (KRFYVVMWKK), which is derived from TSP-1 and -2, in bladder cancer.

MATERIALS AND METHODS:

Two-hundred and six bladder cancer tissues were examined for expression of TSP-1, TSP-2, and 4N1K-peptide by immunohistochemistry. Cancer cell proliferation, apoptosis, angiogenesis and matrix metalloproteinase (MMP)-9 immunoreactivity were also examined.

RESULTS:

Expression of TSP-2 and 4N1K-peptide was negatively associated with T stage, metastasis, and grade. TSP-2 expression was negatively associated with cancer cell proliferation and MMP-9 expression, whereas 4N1K-peptide was significantly associated with apoptosis, angiogenesis, and MMP-9 expression. Multivariate analysis showed that 4N1K-peptide expression was a significant predictor of metastasis (hazard ratio=3.90, p=0.002).

CONCLUSION:

TSP-2 and 4N1K peptide played important roles in malignant aggressiveness and progression of bladder cancer via complex mechanisms involving cell proliferation, apoptosis, angiogenesis, and MMP-9.

• Authors: Yuichiro Nakamura, Yasuyoshi Miyata, Kosuke Takehara, Akihiro Asai, Kensuke Mitsunari, Kyohei Araki, Tomohiro Matsuo, Kojiro Ohba, Hideki Sakai
• Journal: Anticancer research (Anticancer Res) Vol. 39 Issue 5 Pg. 2317-2324 (May 2019) ISSN: 1791-7530 [Electronic] Greece
• PMID: 31092423 (Publication Type: Journal Article)
• Copyright: Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• 4N1K peptide
• Biomarkers, Tumor
• Oligopeptides
• Thrombospondin 1
• Thrombospondins
• thrombospondin 2
• thrombospondin-1, human
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Adult
• Aged
• Aged, 80 and over
• Apoptosis
• Biomarkers, Tumor (genetics)
• Cell Proliferation
• Disease Progression
• Female
• Humans
• Male
• Matrix Metalloproteinase 9 (genetics)
• Middle Aged
• Neoplasm Staging
• Neovascularization, Pathologic (genetics, pathology)
• Oligopeptides (genetics)
• Prognosis
• Thrombospondin 1 (genetics)
• Thrombospondins (genetics)
• Urinary Bladder Neoplasms (genetics, pathology)