Abstract |
Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elasticity increased substantially after low-LET proton beam and persisted long after radiation. This may have significant consequences for the migratory properties of melanoma cells, as well as for the cell susceptibility to therapy.
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Authors | Katarzyna Jasińska-Konior, Olga Wiecheć, Michał Sarna, Agnieszka Panek, Jan Swakoń, Marta Michalik, Krystyna Urbańska, Martyna Elas |
Journal | Scientific reports
(Sci Rep)
Vol. 9
Issue 1
Pg. 7008
(05 07 2019)
ISSN: 2045-2322 [Electronic] England |
PMID | 31065009
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- VIM protein, human
- Vimentin
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Topics |
- Actin Cytoskeleton
(metabolism, radiation effects)
- Cell Line, Tumor
- Cell Movement
(radiation effects)
- Cell Proliferation
(radiation effects)
- Cell Survival
(radiation effects)
- Elasticity
(radiation effects)
- Gene Expression Regulation, Neoplastic
(radiation effects)
- Humans
- Melanoma
(metabolism, radiotherapy)
- Proton Therapy
(methods)
- Skin Neoplasms
(metabolism, radiotherapy)
- Up-Regulation
- Uveal Neoplasms
(metabolism, radiotherapy)
- Vimentin
(metabolism)
- Melanoma, Cutaneous Malignant
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