Acquired N-Linked Glycosylation Motifs in B-Cell Receptors of Primary Cutaneous B-Cell Lymphoma and the Normal B-Cell Repertoire.

Abstract

Primary cutaneous follicle center lymphoma (PCFCL) is a rare mature B-cell lymphoma with an unknown etiology. PCFCL resembles follicular lymphoma (FL) by cytomorphologic and microarchitectural criteria. FL B cells are selected for N-linked glycosylation motifs in their B-cell receptors (BCRs) that are acquired during continuous somatic hypermutation. The stimulation of mannosylated BCR by lectins on the tumor microenvironment is therefore a candidate driver in FL pathogenesis. We investigated whether the same mechanism could play a role in PCFCL pathogenesis. Full-length functional variable, diversity, and joining gene sequences of 18 PCFCL and 8 primary cutaneous diffuse large B-cell lymphoma, leg-type were identified by unbiased Anchoring Reverse Transcription of Immunoglobulin Sequences and Amplification by Nested PCR and BCR reconstruction from RNA sequencing data. Low BCR variation demonstrated negligible ongoing somatic hypermutation in PCFCL and primary cutaneous diffuse large B-cell lymphoma, leg-type, and indicated that the PCFCL microarchitecture does not act as a functional germinal center. Similar to FL but in contrast to primary cutaneous diffuse large B-cell lymphoma, leg-type, BCR genes of 15 PCFCLs (83%) had acquired N-linked glycosylation motifs. These motifs were located at the BCR positions converted to N-linked glycosylation motifs in normal B-cell repertoires with low prevalence but mostly at different positions than those found in FL. The cutaneous localization of PCFCL might suggest a role for lectins from commensal skin bacteria in PCFCL lymphomagenesis.

• Authors: Marvyn T Koning, Edwin Quinten, Willem H Zoutman, Szymon M Kiełbasa, Hailiang Mei, Cornelis A M van Bergen, Patty Jansen, Rochelle D Vergroesen, Rein Willemze, Maarten H Vermeer, Cornelis P Tensen, Hendrik Veelken
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 139 Issue 10 Pg. 2195-2203 (10 2019) ISSN: 1523-1747 [Electronic] United States
• PMID: 31042459 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Receptors, Antigen, B-Cell

Topics

• Aged
• Aged, 80 and over
• Biopsy, Needle
• Cohort Studies
• DNA Mutational Analysis
• Diagnosis, Differential
• Female
• Gene Expression Regulation
• Germinal Center (metabolism)
• Glycosylation
• Humans
• Immunohistochemistry
• Lymphoma, Follicular (genetics, pathology)
• Lymphoma, Large B-Cell, Diffuse (genetics, pathology)
• Male
• Middle Aged
• Molecular Sequence Data
• Prognosis
• Receptors, Antigen, B-Cell (genetics)
• Skin Neoplasms (genetics, pathology)
• Tumor Microenvironment (genetics)