[CXCL13 promotes proliferation and autophagy of human mesenchymal stem cells through MAPK signaling pathway under hypoxic condition].

Abstract

Objective To explore the possible effect of C-X-C motif chemokine ligands13 (CXCL13) on the autophagy and proliferation of human mesenchymal stem cells (hBMSCs) and the underlying mechanisms. Methods The hBMSCs were divided into control group, hypoxia group, CXCL13 treatment group, and CXCL13 combined with SB203580 group. Except the control group, the rest of cells were cultured under the established hypoxia condition of 920 mL/L N2, 30 mL/L O2 and 50 mL/L CO2. The cells of CXCL13 treatment group were stimulated with 50 ng/mL recombinant CXCL13. In the combination group, the cells were preincubated with 20 μmol /L of MAPK inhibitor SB203580 for 30 minutes and subsequently treated with 50 ng/mL recombinant CXCL13. MTT assay was used to determine the proliferation of hBMSCs. The apoptosis rate of hBMSCs was analyzed by annexin V-FITC/PI double labeling and flow cytometry. The protein levels of nuclear factor-κB (NF-κB) and beclin-1 were measured by Western blot analysis. ELISA was used to measure the content of autophagy-related protein 7 (ATG7) in cell lysis buffer. Results Compared with the hypoxia group and the combination group. The cell proliferation, beclin-1 and NF-κB protein levels increased significantly in the CXCL13 treatment group however, the apoptosis rate decreased, and the level of ATG7 increased significantly in cell lysate. Conclusion Under hypoxic condition, CXCL13 promotes autophagy and proliferation of hBMSCs through the MAPK/NF-κB signaling pathway.

• Authors: Yongtao Li, Lulu Wang, Lijie Yao, Shizhu Sun, Lei Shen
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 35 Issue 3 Pg. 211-216 (Mar 2019) ISSN: 1007-8738 [Print] China
• PMID: 31030713 (Publication Type: Journal Article)

Chemical References

• CXCL13 protein, human
• Chemokine CXCL13

Topics

• Apoptosis
• Autophagy
• Cell Hypoxia
• Cell Proliferation
• Chemokine CXCL13
• Humans
• MAP Kinase Signaling System
• Mesenchymal Stem Cells