Structural characterization and rheological properties of a pectin with anti-constipation activity from the roots of Arctium lappa L.

Abstract

A new pectin (ALP-2) was extracted from the roots of Arctium lappa L. with the molecular weight of 1.84 × 106 Da. ALP-2 was composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose. Results of NMR revealed that the dominant linkage types of ALP-2 were →4-α-GalpA-6-OMe-(1→, →2-α-Rha-(1→, →5-α-Araf-(1→ and →3,6-β-Galp-(1→. The ELISA results indicated ALP-2 was a typical pectin with HG chain and RG-I chain. The rheological experiments showed that ALP-2 fluid exhibited shear thinning behavior. The viscosity of ALP-2 was mainly affected by concentration, temperature, and pH. The ALP-2 fluid with elastic properties at high frequencies could be used as a thickener in the food industry. Moreover, ALP-2 with the dosages of 200 mg/kg and 400 mg/kg exhibited strong anti-constipation activity in vivo. ALP-2 treated groups could improve small intestinal movement rate and increase the weight of feces significantly in constipation mice. Therefore, ALP-2 could be considered as the active component for functional food or therapeutic agent in constipation therapy.

• Authors: Kaidong Li, Lingling Zhu, Huan Li, Yiqing Zhu, Chun Pan, Xiangdong Gao, Wei Liu
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 215 Pg. 119-129 (Jul 01 2019) ISSN: 1879-1344 [Electronic] England
• PMID: 30981336 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Laxatives
• Pectins

Topics

• Animals
• Arctium (chemistry)
• Constipation (drug therapy)
• Gastrointestinal Motility (drug effects)
• Intestine, Small (drug effects)
• Laxatives (chemistry, isolation & purification, pharmacology, therapeutic use)
• Mice
• Mice, Inbred ICR
• Pectins (chemistry, isolation & purification, pharmacology, therapeutic use)
• Plant Roots (chemistry)
• Rheology