Effect of bronchial thermoplasty on structural changes and inflammatory mediators in the airways of subjects with severe asthma.

Abstract

BACKGROUND:

Bronchial thermoplasty (BT) is a novel technique used in the treatment of subjects with severe refractory asthma. Radiofrequency is provided to airway walls during bronchoscopy in order to reduce airway remodeling. Several clinical studies have reported an improvement in subjects' symptoms following BT. However, how BT affects the airway architectures and inflammatory mediators in the airways has not been yet fully elucidated.

METHODS:

Fourteen subjects with severe asthma were recruited in this study according to the criteria of ATS severe asthma definition. The study subjects undertook bronchial biopsy during the bronchoscopy procedure at baseline and 6 weeks after the initial BT treatment. The obtained samples were stained with antibodies for α-smooth muscle actin (α-SMA); protein gene product (PGP) 9.5, a specific nerve marker; von Willebrand factor (vWF), a marker for blood vessels; interleukin-17A (IL-17A) and transforming growth factor-β1 (TGF-β1).

RESULTS:

The expression of α-SMA and PGP9.5 were significantly reduced post-BT. There was no significant difference in the number of blood vessels between baseline and post-BT. In addition, BT did not affect the production of IL-17A and TGF-β1 in the airways. The changes in the expression of α-SMA and PGP9.5 had no significant correlation with the improvement of pulmonary function.

CONCLUSION:

and Clinical Relevance: This study suggests that BT reduces airway smooth muscle mass and the airway innervation without affecting vasculature and the production of inflammatory mediators in the airways of subjects with severe asthma.

• Authors: Tomohiro Ichikawa, Alice Panariti, Severine Audusseau, Andrea Karen Mogas, Ronald Olivenstein, Jamila Chakir, Michel Laviolette, Zoulfia Allakhverdi, Saba Al Heialy, James G Martin, Qutayba Hamid
• Journal: Respiratory medicine (Respir Med) Vol. 150 Pg. 165-172 (04 2019) ISSN: 1532-3064 [Electronic] England
• PMID: 30961946 (Publication Type: Clinical Trial, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• ACTA2 protein, human
• Actins
• Inflammation Mediators
• Interleukin-17
• Proteins
• Transforming Growth Factor beta1
• von Willebrand Factor

Topics

• Actins (metabolism, radiation effects)
• Adult
• Airway Remodeling (radiation effects)
• Asthma (therapy)
• Biopsy
• Bronchi (pathology)
• Bronchial Thermoplasty (adverse effects, methods)
• Bronchoscopy (methods)
• Female
• Humans
• Inflammation Mediators (metabolism, radiation effects)
• Interleukin-17 (metabolism, radiation effects)
• Male
• Middle Aged
• Proteins (metabolism, radiation effects)
• Radiofrequency Therapy (methods)
• Respiratory Function Tests (statistics & numerical data)
• Severity of Illness Index
• Transforming Growth Factor beta1 (metabolism, radiation effects)
• von Willebrand Factor (metabolism, radiation effects)