Abstract |
To develop a practically applicable method for T-cell receptor (TCR)-engineered T cell immunotherapy targeting neoantigens, we have been attempting to identify neoantigen-specific T cell receptors (TCRs) and establish TCR-engineered T cells in a 3-4-month period. In this study, we report the characterization of T cell repertoires in tumor microenvironment (TME) and identification of neoantigen-specific TCRs after stimulation of patient-derived T cells. We screened 15 potential neoantigen peptides and successfully identified two CD8+HLA-dextramer+ T cells, which recognized MAGOHBG17A and ZCCHC14P368L. All three dominant TCR clonotypes from MAGOHBG17A-HLA dextramer-sorted CD8+ T cells were also found in T cells in TME, while none of dominant TCR clonotypes from ZCCHC14P368L-HLA dextramer-sorted CD8+ T cells was found in the corresponding TME. The most dominant TCRA/TCRB pairs for these two neoantigens were cloned into HLA-matched healthy donors' T lymphocytes to generate TCR-engineered T cells. The functional assay showed MAGOHBG17A TCR-engineered T cells could be significantly activated in a mutation-specific, HLA-restricted and peptide-dose-dependent manner while ZCCHC14P368L TCR-engineered T cells could not. Our data showed neoantigen-reactive T cell clonotypes that were identified in the patient's peripheral blood could be present in the corresponding TME and might be good TCRs targeting neoantigens.
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Authors | Lili Ren, Matthias Leisegang, Boya Deng, Tatsuo Matsuda, Kazuma Kiyotani, Taigo Kato, Makiko Harada, Jae-Hyun Park, Vassiliki Saloura, Tanguy Seiwert, Everett Vokes, Nishant Agrawal, Yusuke Nakamura |
Journal | Oncoimmunology
(Oncoimmunology)
2019
Vol. 8
Issue 4
Pg. e1568813
ISSN: 2162-4011 [Print] United States |
PMID | 30906664
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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