Combined use of Serum miR-338-3p, Cartilage Oligomeric Matrix Protein and Chondroitin Sulfate-846 in the Early Diagnosis of Knee Osteoarthritis.

Abstract

BACKGROUND:

In this study, the serum levels of miR-338-3p, COMP, and CS-846 were detected in the patients with KOA and healthy controls, to explore the value of miR-338-3p, COMP, and CS-846 in the early diagnosis of KOA.

METHODS:

Real-time PCR was carried out to evaluate the level of miR-338-3p in KOA patients and healthy controls. Spearman's correlation coefficient was performed to examine the correlation between the expression of miR-338-3p, COMP, and CS-846. Receiver operating characteristic (ROC) curves were carried out to evaluate the diagnostic values of miR-338-3p, COMP, and CS-846 for KOA patients.

RESULTS:

In the current study, we first demonstrated that serum miR-338-3p, COMP, and CS-846 levels were increased in KOA patients compared to healthy controls. Moreover, the increase of miR-338-3p, COMP, and CS-846 levels positively correlated with VAS scores and joint space narrowing, suggesting miR-338-3p positively correlated with comprehensive disease severity. In addition, miR-338-3p, COMP, and CS-846 could be used as an independent biomarker for KOA patients. More importantly, combined use of miR-338-3p, COMP, and CS-846 demonstrates a higher diagnostic value with an AUC 0.926 for KOA patients.

CONCLUSIONS:

The combination of miR-338-3p, COMP, and CS-846 demonstrated higher diagnostic value for KOA patients, indicating their combination as novel and promising biomarkers for diagnosis and disease severity of KOA.

• Authors: Xiaojie Dou, Zhaodong Zhang, Shuai Wang, Xuemei Zhou
• Journal: Clinical laboratory (Clin Lab) Vol. 65 Issue 3 (Mar 01 2019) ISSN: 1433-6510 [Print] Germany
• PMID: 30868862 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• COMP protein, human
• Cartilage Oligomeric Matrix Protein
• MIRN338 microRNA, human
• MicroRNAs
• Chondroitin Sulfates

Topics

• Aged
• Aged, 80 and over
• Biomarkers (blood)
• Cartilage Oligomeric Matrix Protein (blood)
• Case-Control Studies
• Chondroitin Sulfates (blood)
• Early Diagnosis
• Humans
• MicroRNAs (blood)
• Middle Aged
• Osteoarthritis, Knee (blood, diagnosis)