Synergistically dual-functional nano eye-drops for simultaneous anti-inflammatory and anti-oxidative treatment of dry eye disease.

Abstract

We have developed a rapid and straightforward topical treatment method for dry eye disease (DED) using poly(catechin) capped-gold nanoparticles (Au@Poly-CH NPs) carrying amfenac [AF; a nonsteroidal anti-inflammatory drug (NSAID)] through effective attenuation of ocular surface tissue damage in dry eyes. A dual-targeted strategy based on ocular therapeutics was adopted to simultaneously block the cyclooxygenase enzymes-induced inflammation and reactive oxygen species (ROS)-induced oxidative stress, the primary two causes of DED. The self-assembled core-shell Au@Poly-CH NPs synthesized via a simple reaction between tetrachloroaurate(iii) and catechin possess a poly(catechin) shell (∼20 nm) on the surface of each Au NP (∼60 nm). The anti-oxidant and anti-inflammatory properties of AF/Au@Poly-CH NPs were evaluated by DCFH-DA and prostaglandin E2/VEGF assays, respectively. Our results demonstrate that Au@Poly-CH NPs not only act as an anti-oxidant to suppress ROS-mediated processes, but also serve as a drug carrier of AF for a synergistic effect on anti-inflammation. In vivo biocompatibility studies show good tolerability of AF/Au@Poly-CH NPs for potential use in the treatment of ocular surface pathologies. The dual-targeted therapeutic effects of AF/Au@Poly-CH NPs lead to rapid recovery from DED in a rabbit model. Au@Poly-CH NPs loaded with NSAIDs is a promising multifunctional nanocomposite for treating various inflammation- and oxidative stress-related diseases.

• Authors: Yu-Jia Li , Li-Jyuan Luo , Scott G Harroun , Shih-Chun Wei , Binesh Unnikrishnan , Huan-Tsung Chang , Yu-Fen Huang , Jui-Yang Lai , Chih-Ching Huang
• Journal: Nanoscale (Nanoscale) Vol. 11 Issue 12 Pg. 5580-5594 (Mar 21 2019) ISSN: 2040-3372 [Electronic] England
• PMID: 30860532 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Biocompatible Materials
• Mucin 5AC
• Ophthalmic Solutions
• Phenylacetates
• Reactive Oxygen Species
• Vascular Endothelial Growth Factor A
• amfenac
• Gold
• Catechin
• Prostaglandin-Endoperoxide Synthases

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology, therapeutic use)
• Antioxidants (chemistry)
• Biocompatible Materials (chemistry)
• Catechin (chemistry)
• Cell Line
• Cornea (cytology, metabolism, pathology)
• Drug Liberation
• Dry Eye Syndromes (drug therapy, pathology)
• Gold (chemistry)
• Metal Nanoparticles (chemistry)
• Microscopy, Fluorescence
• Mucin 5AC (metabolism)
• Ophthalmic Solutions (chemistry, pharmacology, therapeutic use)
• Oxidative Stress (drug effects)
• Phenylacetates (chemistry, pharmacology, therapeutic use)
• Prostaglandin-Endoperoxide Synthases (chemistry, metabolism)
• Rabbits
• Reactive Oxygen Species (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)