Studies on formulation of a combination heat killed immunogen from diarrheagenic Escherichia coli and Vibrio cholerae in RITARD model.

Abstract

Multiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we formulated a combination of antigens from three different diarrheagenic Escherichia coli strains and three different Vibrio cholerae strains. We demonstrated that our newly formulated combination immunogen was able to raise species-specific immunogenicity. This formulation also gave protection against different diarrheagenic E. coli strains in the removable intestinal tie-adult rabbit diarrhea model. However, protective efficacy was not found against the V. cholerae El Tor Ogawa Haitian variant, but challenged with V. cholerae El Tor Inaba or O139 showed protection in rabbits. This is the first report of a single formulated nonliving heat-killed combination immunogen from different diarrheagenic E. coli and V. cholerae that could bestow protection against different bacteria in an animal model.

• Authors: Priyadarshini Mukherjee, Vivek Mondal, Ushasi Bhaumik, Ritam Sinha, Sounak Sarkar, Soma Mitra, Debaki Ranjan Howlader, Suhrid Maiti, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta Koley
• Journal: Microbes and infection (Microbes Infect) 2019 Oct - Nov Vol. 21 Issue 8-9 Pg. 368-376 ISSN: 1769-714X [Electronic] France
• PMID: 30853357 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Cholera Vaccines
• Escherichia coli Vaccines
• Immunoglobulins
• Vaccines, Combined
• Vaccines, Inactivated

Topics

• Animals
• Antibodies, Bacterial (blood, immunology)
• Antigens, Bacterial (immunology)
• Cholera (prevention & control)
• Cholera Vaccines (administration & dosage, immunology)
• Cross Protection (immunology)
• Diarrhea (microbiology, prevention & control)
• Disease Models, Animal
• Escherichia coli (genetics, immunology)
• Escherichia coli Infections (prevention & control)
• Escherichia coli Vaccines (administration & dosage, immunology)
• Immunization
• Immunoglobulins (blood)
• Rabbits
• Vaccines, Combined (immunology)
• Vaccines, Inactivated (immunology)
• Vibrio cholerae (genetics, immunology)