Abstract | PURPOSE: Optical redox imaging (ORI), based on collecting the endogenous fluorescence of reduced nicotinamide adenine dinucleotide ( NADH) and oxidized flavoproteins (Fp) containing a redox cofactor flavin adenine dinucleotide ( FAD), provides sensitive indicators of cellular metabolism and redox status. ORI indices (such as NADH, FAD, and their ratio) have been under investigation as potential progression/prognosis biomarkers for cancer. Higher FAD redox ratio (i.e., FAD/( FAD + NADH)) has been associated with higher invasive/metastatic potential in tumor xenografts and cultured cells. This study is to examine whether ORI indices can respond to the modulation of oncogene DEK activities that change cancer cell invasive/metastatic potential. PROCEDURES: Using lentiviral shRNA, DEK gene expression was efficiently knocked down in MDA-MB-231 breast cancer cells (DEKsh). These DEKsh cells, along with scrambled shRNA-transduced control cells (NTsh), were imaged with a fluorescence microscope. In vitro invasive potential of the DEKsh cells and NTsh cells was also measured in parallel using the transwell assay. RESULTS:
FAD and FAD redox ratios in polyclonal cells with DEKsh were significantly lower than that in NTsh control cells. Consistently, the DEKsh cells demonstrated decreased invasive potential than their non-knockdown counterparts NTsh cells. CONCLUSIONS: This study provides direct evidence that oncogene activities could mediate ORI-detected cellular redox state.
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Authors | Yu Wen, He N Xu, Lisa Privette Vinnedge, Min Feng, Lin Z Li |
Journal | Molecular imaging and biology
(Mol Imaging Biol)
Vol. 21
Issue 3
Pg. 410-416
(06 2019)
ISSN: 1860-2002 [Electronic] United States |
PMID | 30758703
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Chromosomal Proteins, Non-Histone
- DEK protein, human
- Oncogene Proteins
- Poly-ADP-Ribose Binding Proteins
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Topics |
- Breast Neoplasms
(diagnostic imaging, metabolism, pathology)
- Cell Line, Tumor
- Chromosomal Proteins, Non-Histone
(genetics)
- Female
- Gene Knockdown Techniques
- Humans
- Neoplasm Invasiveness
- Oncogene Proteins
(genetics)
- Oncogenes
- Optical Imaging
- Oxidation-Reduction
- Poly-ADP-Ribose Binding Proteins
(genetics)
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