RNA-Seq profiling in peripheral blood mononuclear cells of amyotrophic lateral sclerosis patients and controls.

Abstract

Coding and long non-coding RNA (lncRNA) metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we present a dataset obtained via Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1 and VCP genes) and healthy controls. This dataset allows the whole-transcriptome characterization of PBMCs content, both in terms of coding and non-coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Our dataset is a starting point for the omni-comprehensive analysis of coding and lncRNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.

• Authors: Susanna Zucca, Stella Gagliardi, Cecilia Pandini, Luca Diamanti, Matteo Bordoni, Daisy Sproviero, Maddalena Arigoni, Martina Olivero, Orietta Pansarasa, Mauro Ceroni, Raffaele Calogero, Cristina Cereda
• Journal: Scientific data (Sci Data) Vol. 6 Pg. 190006 (02 05 2019) ISSN: 2052-4463 [Electronic] England
• PMID: 30720798 (Publication Type: Dataset, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• FUS protein, human
• RNA, Long Noncoding
• RNA-Binding Protein FUS
• SOD1 protein, human
• TARDBP protein, human
• Superoxide Dismutase-1
• VCP protein, human
• Valosin Containing Protein

Topics

• Amyotrophic Lateral Sclerosis (genetics)
• DNA-Binding Proteins (genetics)
• Gene Expression Profiling
• Humans
• Leukocytes, Mononuclear (metabolism)
• Mutation
• RNA, Long Noncoding (genetics, physiology)
• RNA-Binding Protein FUS (genetics)
• Superoxide Dismutase-1 (genetics)
• Valosin Containing Protein (genetics)