The symbiotic
infection of root cells by
nitrogen-fixing rhizobia during nodulation requires the
transcription factor Nodule Inception (NIN). Our root hair transcriptomic study extends NIN's regulon to include Rhizobium Polar Growth and genes involved in cell wall modification,
gibberellin biosynthesis, and a comprehensive group of nutrient (N, P, and S) uptake and assimilation genes, suggesting that NIN's recruitment to nodulation was based on its role as a growth module, a role shared with other NIN-Like
Proteins. The expression of
jasmonic acid genes in nin suggests the involvement of NIN in the resolution of growth versus defense outcomes. We find that the regulation of the growth module component Nodulation
Pectate Lyase by NIN, and its function in rhizobial
infection, are conserved in hologalegina legumes, highlighting its recruitment as a major event in the evolution of nodulation. We find that Nodulation
Pectate Lyase is secreted to the
infection chamber and the lumen of the
infection thread. Gene network analysis using the
transcription factor mutants for ERF Required for Nodulation1 and
Nuclear Factor-Y Subunit A1 confirms hierarchical control of NIN over
Nuclear Factor-Y Subunit A1 and shows that ERF Required for Nodulation1 acts independently to control infection. We conclude that while NIN shares functions with other NIN-Like
Proteins, the conscription of key
infection genes to NIN's control has made it a central regulatory hub for rhizobial
infection.