The endothelium plays an important role in
cancer metastasis, but the mechanisms involved are still not clear. In the present work, we characterised the changes in endothelial function at early and late stages of
breast cancer progression in an orthotopic model of murine mammary
carcinoma (4T1 cells). Endothelial function was analysed based on simultaneous microflow liquid chromatography-tandem mass spectrometry using multiple reaction monitoring (microLC/MS-MRM) quantification of 12 endothelium-related
biomarkers, including those reflecting glycocalyx disruption -
syndecan-1 (SDC-1), endocan (ESM-1); endothelial
inflammation -
vascular cell adhesion molecule 1 (VCAM-1),
intercellular adhesion molecule 1 (ICAM-1),
E-selectin (E-sel); endothelial permeability -
fms-like tyrosine kinase 1 (FLT-1),
angiopoietin 2 (Angpt-2); and haemostasis -
von Willebrand factor (vWF),
tissue plasminogen activator (t-PA),
plasminogen activator inhibitor 1 (PAI-1), as well as those that are pathophysiologically linked to endothelial function -
adrenomedullin (ADM) and
adiponectin (ADN). The early phase of
metastasis in mouse plasma was associated with glycocalyx disruption (increased SDC-1 and ESM-1), endothelial
inflammation [increased soluble
VCAM-1 (sVCAM-1)] and increased vascular permeability (Angpt-2). During the late phase of
metastasis, additional alterations in haemostasis (increased PAI-1 and vWF), as well as a rise in ADM and substantial fall in ADN concentration, were observed. In conclusion, in a murine model of
breast cancer metastasis, we identified glycocalyx disruption, endothelial
inflammation and increased endothelial permeability as important events in early
metastasis, while the late phase of
metastasis was additionally characterised by alterations in haemostasis.