One New Phenolic Compound from Castanea mollissima Shells and its Suppression of HepatomaCell Proliferation and Inflammation by Inhibiting NF-κB Pathway.

Abstract

Shells of Castanea mollissima (CMS), an agricultural remain and often considered waste from chestnut processing industry, have been proven a resource for traditional Chinese medicine. One new phenol, named castanolB(1), andsix known phenolic compounds (2⁻7) were isolated froma water-soluble extract of CMS. Their chemical structures were determined using preparative HPLC and various spectral analyses, and then were compared to literatures, which indicated the first identification of the seven compounds from C. mollissima. The physicochemical property of compound (2) was also reported for the first time. After antiproliferative screening of compounds (1⁻7) on LPS-induced SMMC-7721 and HepG2 hepatoma cells, castanolB (1) showed the best suppression. CastanolB(1) also significantly induced cell apoptosis. Furthermore, castanolB (1) decreasedsecretion of TNF-α and IL-6. Mechanistically, TLR4⁻NF-κB pathway was inhibited bycastanolB (1) with downregulation of TLR4, IKKβ, and NF-κB p65. This study presents a new phenol and shows its profiles of anticancer and anti-inflammation via inhibiting the TLR4⁻NF-κB pathway.

• Authors: Wu Fei, Yao Xuan, Xu Jian, Wu Yue, Yang Yuejun, Jin Yu, Xie Huifang, Liu Yuancai, Yang Yifu, Zheng Xiangwei
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 20 Issue 3 (Jan 22 2019) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 30678222 (Publication Type: Journal Article)

Chemical References

• Diterpenes
• I-kappa B Proteins
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Phenols
• Plant Extracts
• Toll-Like Receptor 4
• Tumor Necrosis Factor-alpha
• castanol B

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Diterpenes (pharmacology)
• Fagaceae (chemistry)
• Hep G2 Cells
• Humans
• I-kappa B Proteins (metabolism)
• Inflammation (chemically induced, metabolism)
• Interleukin-6 (metabolism)
• Lipopolysaccharides (toxicity)
• NF-kappa B (metabolism)
• Phenols (pharmacology)
• Plant Extracts (pharmacology)
• Toll-Like Receptor 4 (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)