In vitro activity of flomoxef against extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Korea.

Abstract

To find an alternative regimen for the treatment of extended-spectrum β-lactamase (EBSL)-producing Enterobacteriaceae infections, we examined the in vitro activity of flomoxef against Escherichia coli and Klebsiella pneumoniae having CTX-M-1 group and/or CTX-M-9 group ESBLs. Boronic acid disk methods and polymerase chain reaction amplification were used to detect for ESBL, and AmpC β-lactamase and AmpC β-lactamase co-producers were excluded. Minimum inhibitory concentrations (MICs) were determined for flomoxef by broth microdilution. One hundred seventy-six isolates (E. coli, n = 93 and K. pneumoniae, n = 83) were analyzed for susceptibility test. A total of 94.3% (166/176) of isolates were susceptible to flomoxef (MIC50/MIC90 were 0.5/8 μg/mL); 98.9% of the ESBL-producing E. coli (MIC50/MIC90 were 1/4 μg/mL) and 89.2% of the ESBL-producing K. pneumoniae (MIC50/MIC90 were 0.5/16 μg/mL) were susceptible to flomoxef. Flomoxef has good in vitro activity against ESBL-producing E. coli and K. pneumoniae and could be considered as an alternative for infections caused by these organisms.

• Authors: Younghee Jung, Seung Soon Lee, Wonkeun Song, Han-Sung Kim, Young Uh
• Journal: Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 94 Issue 1 Pg. 88-92 (May 2019) ISSN: 1879-0070 [Electronic] United States
• PMID: 30658867 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Cephalosporins
• Escherichia coli Proteins
• CTX-M-9 protein, E coli
• beta-lactamase TEM-3
• beta-Lactamases
• flomoxef

Topics

• Anti-Bacterial Agents (pharmacology)
• Cephalosporins (pharmacology)
• Escherichia coli (drug effects, enzymology, isolation & purification)
• Escherichia coli Infections (microbiology)
• Escherichia coli Proteins (metabolism)
• Hospitals, University
• Humans
• Klebsiella Infections (microbiology)
• Klebsiella pneumoniae (drug effects, enzymology, isolation & purification)
• Korea
• Microbial Sensitivity Tests
• beta-Lactamases (metabolism)