Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India.

Abstract

BACKGROUND:

The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region.

METHODS:

We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n=70) from New Delhi.

RESULTS:

NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50-1:30,264), binding antibodies (range, 1:3,000-1:134,000,) as well as the MBC (range=0.006%-5.05%).

CONCLUSION:

These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs - emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

• Authors: Sivaram Gunisetty, Kaustuv Nayak, Ramesh Chandra Rai, Yadya Chawla, Elluri Seetharami Reddy, Charu Aggarwal, Deepti Maheshwari, Harekrushna Panda, Nasim Akhtar Ansari, Prabhat Singh, Manpreet Kaur, Kritika Dixit, Pragati Sharma, Priya Bhatnagar, Lalita Priyamvada, Siddhartha Kumar Bhaumik, Syed Fazil Ahamed, Rosario Vivek, Pratima Ray, Anita Shet, Poonam Coshic, Rakesh Lodha, Sushil Kumar Kabra, Dil Afroze, Adfar Yousuf, Rafi Ahmed, Kaja Murali-Krishna, Anmol Chandele
• Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (Int J Infect Dis) Vol. 84S Pg. S57-S63 (Jul 2019) ISSN: 1878-3511 [Electronic] Canada
• PMID: 30658170 (Publication Type: Journal Article)
• Copyright: Copyright © 2019. Published by Elsevier Ltd.

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral

Topics

• Adult
• Antibodies, Neutralizing (immunology)
• Antibodies, Viral (immunology)
• B-Lymphocytes (immunology)
• Cross Reactions
• Dengue (epidemiology, immunology)
• Dengue Virus (immunology)
• Female
• Humans
• India
• Male
• Serogroup
• Young Adult