HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

A simple and high-throughput luciferase immunosorbent assay for both qualitative and semi-quantitative detection of anti-HIV-1 antibodies.

Abstract
In this study, we described an ultrasensitive and high-throughput luciferase immunosorbent assay (LISA) for qualitative and quantitative detection of anti-HIV-1 antibody. Anti-HIV antibody in serum or plasma samples was captured by protein A/G-coated microtiter plate and detected with crude cell lysates expressing Nanoluc luciferase (Nluc) enzyme fused with HIV-1 p24 or gp41 antigen without the need of protein purification. After the addition of furimazine substrate, anti-HIV antibodies were quantitatively measured as luciferase light units. LISA showed a wide linear range of detection and was about 104-fold more sensitive than ELISA. For the detection of both anti-p24 and anti-gp41, LISA showed extraordinary sensitivity (99.5% and 100%, respectively) and equivalent specificity (100%). LISA could also monitor the change in the anti-HIV-1 antibody response over time in antiretroviral therapy (ART) treated individuals, and can sufficiently distinguish between recent and long-term HIV-1 infections. Our preliminary results indicate that LISA may provide a novel universal immunoassay platform for simultaneous HIV-1 detection, quantitative measurement of anti-HIV antibodies as well as the differentiation of HIV-1 infection stages.
AuthorsHaiying Wang, Qundi Cai, Yuanhao Liang, Jingwei Shui, Shixing Tang
JournalVirus research (Virus Res) Vol. 263 Pg. 9-15 (04 02 2019) ISSN: 1872-7492 [Electronic] Netherlands
PMID30605754 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2018. Published by Elsevier B.V.
Chemical References
  • HIV Antibodies
  • HIV Core Protein p24
  • HIV Envelope Protein gp41
  • gp41 protein, Human immunodeficiency virus 1
  • Luciferases
Topics
  • Diagnostic Tests, Routine (methods)
  • HIV Antibodies (blood)
  • HIV Core Protein p24 (immunology)
  • HIV Envelope Protein gp41 (immunology)
  • HIV Infections (diagnosis)
  • HIV-1 (immunology)
  • Immunosorbent Techniques
  • Luciferases (analysis)
  • Luminescent Measurements (methods)
  • Protein Binding
  • Sensitivity and Specificity
  • Staining and Labeling

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: