Etomidate Suppresses Invasion and Migration of Human A549 Lung Adenocarcinoma Cells.

Abstract

BACKGROUND/AIM:

Etomidate, an intravenous anesthetic, has been shown to have anticancer effects, including induction of cell-cycle arrest and apoptosis. However, to our knowledge, there are no reports about the anti-metastasis effects of etomidate on A549 human lung adenocarcinoma cells.

MATERIALS AND METHODS:

The cell viability, cell adhesion, gelatin zymography assay, transwell migration and invasion assay, and western blotting analysis were used to investigate the effects of etomidate on A549 cells.

RESULTS:

In our study, etomidate showed low cytotoxicity, inhibited cell adhesion, and suppressed the migration and invasion in A549 cells. The activity of matrix metallopeptidase 2 (MMP2) was reduced by 48 h treatment of etomidate. Results of western blotting analysis indicated that etomidate down-regulated the expression of protein kinase C, MMP7, MMP1, MMP9, and p-p-38, but up-regulated that of RAS, phosphoinositide 3-kinase, and phosphor-extracellular-signal related kinase after 24 and 48 h treatment, in A549 cells.

CONCLUSION:

Etomidate suppressed the migration and invasion of lung adenocarcinoma A549 cells via inhibiting the expression of MMP1, MMP2, MMP7 and MMP9, and provides potential therapeutic targets for lung cancer treatment.

• Authors: Chin-Nan Chu, King-Chuen Wu, Wai-Shan Chung, Li-Cheng Zheng, Ta-Kuo Juan, Yung-Ting Hsiao, Shu-Fen Peng, Jung-Long Yang, Yi-Shih Ma, Rick Sai-Chuen Wu, Jing-Gung Chung
• Journal: Anticancer research (Anticancer Res) Vol. 39 Issue 1 Pg. 215-223 (Jan 2019) ISSN: 1791-7530 [Electronic] Greece
• PMID: 30591461 (Publication Type: Journal Article)
• Copyright: Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Neoplasm Proteins
• Metalloendopeptidases
• Etomidate

Topics

• A549 Cells
• Adenocarcinoma of Lung (drug therapy, genetics, pathology)
• Apoptosis (drug effects)
• Cell Adhesion (drug effects)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Etomidate (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Metalloendopeptidases (genetics)
• Neoplasm Invasiveness (genetics)
• Neoplasm Proteins (genetics)