Effect of oral magnesium sulfate administration on blood glucose hemostasis via inhibition of gluconeogenesis and FOXO1 gene expression in liver and muscle in diabetic rats.

Abstract

The present study was designed to investigate the possible role of Mg2+ in suppression of phosphoenolpyruvate carboxy kinase (PEPCK) enzyme via inhibition of FOXO1gene expression in liver and we also examined whether Mg contributes to decrease blood glucose in muscle via inhibiting FOXO1 gene and protein expression. Fifty rats in five groups of experiment were considered as; non-diabetic control (NDC), Mg2+-treated non-diabetic control (Mg2+-NDC), chronic diabetic (CD), Mg2+-treated chronic diabetic (Mg2+-CD), and insulin-treated chronic diabetic (Ins-CD). Streptozotocin (STZ) was used for diabetes induction. The Mg2+-CD and Mg2+-NDC groups received 10 g/l of magnesium sulfate (MgSO4) added to drinking water, and Ins-CD group received 2.5 U/kg of insulin. The blood glucose level and body weight were measured weekly. After 16 weeks, intraperitoneal glucose tolerance test (IPGTT) was done and blood samples were taken to determine the plasma levels of Mg and gastrocnemius muscle legs, and liver were isolated for both Forkhead transcription factor (FOXO1) and PEPCK enzyme genes and proteins expression. Administration of MgSO4 improved IPGTT, lowered blood glucose levels and decreased FOXO1 and PEPCK genes and proteins expression in muscle and liver, while insulin just could decrease FOXO1 gene and protein expression in the muscle. These findings illustrated that MgSO4 improved hyperglycemia via inhibition of FOXO1 gene and protein level in the muscle and liver, and it also decreased blood glucose level by prohibition of gluconeogenesis pathway in the liver. However, long time administration of insulin did not have any effect on liver.

• Authors: Ayeshe Barooti, Mitra Kamran, Fatemeh Kharazmi, Ebrahim Eftakhar, Kianoosh Malekzadeh, Ardeshir Talebi, Nepton Soltani
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 109 Pg. 1819-1825 (Jan 2019) ISSN: 1950-6007 [Electronic] France
• PMID: 30551436 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Masson SAS.

Chemical References

• Blood Glucose
• Insulin
• Nerve Tissue Proteins
• Foxo1 protein, rat
• Streptozocin
• Magnesium Sulfate
• Glucose

Topics

• Administration, Oral
• Animals
• Blood Glucose (drug effects)
• Diabetes Mellitus, Experimental (chemically induced, drug therapy, metabolism)
• Gene Expression (drug effects)
• Gluconeogenesis (drug effects)
• Glucose (metabolism)
• Glucose Tolerance Test (methods)
• Hemostasis (drug effects)
• Hyperglycemia (drug therapy, metabolism)
• Insulin (metabolism)
• Insulin Resistance (physiology)
• Liver (embryology, metabolism)
• Magnesium Sulfate (administration & dosage)
• Male
• Muscles (drug effects, metabolism)
• Nerve Tissue Proteins (genetics)
• Rats
• Rats, Wistar
• Streptozocin (pharmacology)