Geraniol protects against lipopolysaccharide and D-galactosamine-induced fulminant hepatic failure by activating PPARγ.

Abstract

Geraniol (GOH), a natural component of plant essential oils, exhibits potent antioxidant and anti-inflammatory properties. The aim of this study was to assess the protective effects and mechanisms of GOH on lipopolysaccharide (LPS)/d-galactosamine (D-GalN)-induced fulminant hepatic failure (FHF). Mice were treated with GOH (12.5, 25, and 50 μg/kg) 1 h before challenging LPS (60 mg/kg) and D-GalN (800 mg/kg). 8 h later LPS/D-GlaN treatment, mice were sacrificed and the serum and the liver tissues were collected for testing. The liver pathological changes were assessed by H & E staining. MPO activity, MDA level in liver tissues, and AST, ALT levels in serum were detected by specific detection kits. The levels of TNF-α and IL-1β were detected by ELISA. The expression of NF-κB and PPARγ were detected by western blot analysis and qRT-PCR. The results showed that GOH had a protective effect on LPS/D-GalN-induced FHF, as evidence by the attenuation of liver pathological injury, MPO activity, MDA level, and serum AST and ALT levels. GOH reduced liver TNF-α and IL-1β levels through inhibiting NF-κB signaling pathway activation. Furthermore, GOH increased PPARγ expression in FHF induced by LPS/D-GalN. In conclusion, the present study proved that GOH protects against LPS/D-GalN-induced FHF through inhibiting inflammatory response and increasing PPARγ expression.

• Authors: Yi Li, Nian Wang, Yongfang Jiang
• Journal: Microbial pathogenesis (Microb Pathog) Vol. 128 Pg. 7-12 (Mar 2019) ISSN: 1096-1208 [Electronic] England
• PMID: 30550845 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Ltd.

Chemical References

• Acyclic Monoterpenes
• Anti-Inflammatory Agents
• Antioxidants
• Interleukin-1beta
• Lipopolysaccharides
• NF-kappa B
• Oils, Volatile
• PPAR gamma
• Plant Oils
• Protective Agents
• Terpenes
• Tumor Necrosis Factor-alpha
• Galactosamine
• geraniol

Topics

• Acyclic Monoterpenes
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Disease Models, Animal
• Galactosamine (adverse effects)
• Interleukin-1beta (metabolism)
• Lipopolysaccharides (adverse effects)
• Liver (chemistry, injuries, pathology)
• Liver Failure, Acute (drug therapy)
• Male
• Mice
• Mice, Inbred C57BL
• NF-kappa B (metabolism)
• Oils, Volatile (pharmacology)
• PPAR gamma (metabolism)
• Plant Oils (pharmacology)
• Protective Agents (pharmacology)
• Signal Transduction (drug effects)
• Terpenes (administration & dosage, pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)