Antiserum to endotoxin in hemorrhagic shock.

Abstract

Antiserum to Escherichia coli J5, a mutant endotoxin (LPS) which contains only core determinants, has proven effective in reducing mortality from endotoxic shock due to a wide variety of gram-negative bacteria. Twenty New Zealand white rabbits with coliforms in the gut were subjected to hemorrhagic shock of 36 mm Hg for 3 hr. Treated rabbits were resuscitated with 15 cc of rabbit J5 antiserum (hemagglutinating antibody titer against J5 lipopolysaccharide of 1:1024), remaining shed blood, and lactated Ringer's to achieve a mean arterial blood pressure (MABP) within 20% of baseline. The control group was similarly resuscitated but received 15 cc normal rabbit serum (titer 1:2). Catheters were removed and rabbits were returned to their cages until death or 5 days of survival. Hemodynamic parameters (heart rate, MABP, cardiac output, and total peripheral resistance) did not differ significantly between groups. However, six treated rabbits survived 5 days (60%) and no control rabbit lived past the third postexperimental day (P less than 0.019). Our data suggest that systemic endotoxemia may contribute to morbidity and mortality in severe hemorrhagic shock.

• Authors: E C Pohlson, A Suehiro, E J Ziegler, G Suehiro, J J McNamara
• Journal: The Journal of surgical research (J Surg Res) Vol. 45 Issue 5 Pg. 467-71 (Nov 1988) ISSN: 0022-4804 [Print] United States
• PMID: 3054323 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Endotoxins
• Immune Sera
• Alanine Transaminase

Topics

• Alanine Transaminase (metabolism)
• Animals
• Endotoxins (immunology)
• Escherichia coli (immunology)
• Immune Sera (immunology)
• Immunotherapy
• Rabbits
• Shock, Hemorrhagic (enzymology, mortality, therapy)
• Time Factors