DJ-1 Alters Epirubicin-induced Apoptosis via Modulating Epirubicinactivated Autophagy in Human Gastric Cancer Cells.

Abstract

Epirubicin, which is a conventional chemotherapeutic drug for gastric cancer, has innate and adaptive chemoresistance. Recent studies revealed that epirubicin could induce autophagy as a defensive mechanism in drug resistance of mammary carcinoma. Another study implied that DJ-1 may be a chemoresistance-related gene. But the association between DJ-1 and drug resistance of epirubicin in gastric cancer is still ambiguous. In the present report, we explored whether and how DJ-1 conduced to epirubicin-induced apoptosis in gastric cancer. Epirubicin dose-dependently increased the expression of DJ-1 and induced autophagy. Knockdown of DJ-1 notably enhanced epirubicin-induced cell apoptosis, whereas overexpression of DJ-1 attenuated epirubicin-induced cell apoptosis. Further studies revealed that down-regulation of DJ-1 modulated epirubicinactivated autophagy which augmented epirubicin-induced apoptosis. In conclusion, our results validated that DJ-1 reduced epirubicin-induced apoptosis in gastric cancer cells via modulating epirubicin-activated autophagy.

• Authors: Xue-Kai Pan, Fei Su, Li-Hua Xu, Zhang-Shuo Yang, Dan-Wen Wang, Li-Jie Yang, Fan-Zheng Kong, Wei Xie, Mao-Hui Feng
• Journal: Current medical science (Curr Med Sci) Vol. 38 Issue 6 Pg. 1018-1024 (Dec 2018) ISSN: 2523-899X [Electronic] China
• PMID: 30536064 (Publication Type: Journal Article)

Chemical References

• Epirubicin
• PARK7 protein, human
• Protein Deglycase DJ-1

Topics

• Apoptosis (drug effects)
• Autophagy (drug effects)
• Cell Line, Tumor
• Drug Resistance, Neoplasm (drug effects)
• Epirubicin (pharmacology)
• Humans
• Protein Deglycase DJ-1 (metabolism)
• Stomach Neoplasms (drug therapy, metabolism, pathology)