Cisplatin (cisPt), among the best known components of multi-
drug front-line
therapies used for the treatments of solid
tumors, such as the childhood
neuroblastoma, acts through
DNA linking. Nevertheless, the cisPt effectiveness is compromised by the onset of severe side effects, including neurotoxicity that results in neurodegeneration, cell death, and drug-resistance. In the field of experimental oncology, aimed at overcoming cytotoxicity and chemoresistance, great efforts are devoted to the synthesis of new
platinum-based drugs, such as [Pt(O,O'-acac)(γ-acac)(DMS)] (
PtAcacDMS), which shows a specific reactivity with
sulfur residues of
enzymes involved in apoptosis. Autophagy, an evolutionary conserved degradation pathway for recycling of cytoplasmic components, represents one of the mechanisms adopted by
cancer cells which contribute to drug-resistance. In the present study, standard acute (48 h-exposure) and long-term effects (7 day-recovery
after treatment or 7 day-recovery followed by reseeding and 96 h-growth), of cisPt and
PtAcacDMS (40 and 10 μM, respectively) were investigated in vitro employing rat B50
neuroblastoma as a
cancer model. Using fluorescence and electron microscopy, as well as biochemical techniques, our data highlight a key role of the autophagic process in B50 cells. Specifically, long-term effects caused by cisPt lead to inhibition of the apoptotic process and paralleled by the activation of autophagy, thus evidencing that autophagy has a protective role after cisPt exposure, allowing cells to survive. Whereas, long-term effects produced by
PtAcacDMS lead toward both apoptosis and autophagy activation. In conclusion, autophagy may represents an alternative cell death pathway, circumventing drug-resistance strategies employed by
cancer cells to survive chemoterapy.