Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway.

Abstract

Rheumatoid arthritis (RA) represents a type of autoimmune disease that mainly affect the joints due to persistent synovitis. Eosinophils were Th2 effector cells that have been shown to have anti-inflammatory role recently. In this study, we aimed to investigate the effects of eosinophils transfer on arthritis and underlying mechanisms. DBA/1 mice were induced with collagen-induced arthritis (CIA) and treated with purified eosinophils at different time points. We showed that eosinophils transfer attenuated arthritis in CIA mice. Meanwhile, TNF-α, IL-6, IL-12 and iNOS levels were decreased whereas TGF-β, IL-10, IL-13 and Arg1 levels were increased after eosinophil transfer. In vitro stimulation of bone marrow-derived macrophage (BMDM) with LPS and IFN-γ induced high expression of CD68, iNOS, TNF-α, IL-6, and IL-12, while treatment with eosinophils downregulated their expression levels. Furthermore, high levels of p-IκB and p-P38 expression in BMDM induced by LPS and IFN-γ could be suppressed by eosinophil treatment, and a P38 or IκB inhibitor accelerated the effect of eosinophils on macrophage polarization. Our results demonstrate that eosinophils exert anti-inflammatory effects in arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway.

• Authors: Liu Liu, Yuanyuan Zhang, Xu Zheng, Li Jin, Nan Xiang, Min Zhang, Zhu Chen
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 508 Issue 3 Pg. 894-901 (01 15 2019) ISSN: 1090-2104 [Electronic] United States
• PMID: 30528734 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• I-kappa B Proteins
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Arthritis, Experimental (enzymology, immunology, pathology)
• Cells, Cultured
• Cytokines (biosynthesis)
• Eosinophils (immunology, transplantation)
• I-kappa B Proteins (metabolism)
• Joints
• MAP Kinase Signaling System
• Macrophages (enzymology, immunology)
• Male
• Mice, Inbred BALB C
• Mice, Inbred DBA
• p38 Mitogen-Activated Protein Kinases (metabolism)