Could FA-PG-SPIONs act as a hyperthermia sensitizing agent? An in vitro study.

Abstract

The therapeutic effect of polyglycerol coated iron oxide nanoparticles (PG-SPIONs, non-targeted nanoparticles) and folic acid-conjugated polyglycerol coated iron oxide nanoparticles (FA-PG-SPIONs, targeted nanoparticles) in combination with hyperthermia on viability of HeLa cells was investigated. It was observed that coated and uncoated SPIONs have spherical shapes with an average diameter of 17.9 ± 2.85 nm and 5.4 ± 0.75 nm, respectively. The penetration rate for cells treated with targeted nanoparticles was shown to be more than that of non-targeted nanoparticles. Moreover, it was revealed that the treatment of cells with ≥ 50 µg/ml FA-PG-SPIONs in combination with hyperthermia induced cytotoxicity in comparison to control cells. The results also showed that increasing the concentrations of targeted nanoparticles (FA-PG-SPIONs) and heating time would increase the value of thermal enhancement factor (TEF). In contrast, TEF values were not increased with increasing heating time and concentrations of non-targeted nanoparticles (PG-SPIONs). On the other hand, TEF values were increased with increasing concentrations and heating time so that the maximum TEF value was obtained at the highest concentration (FA-PG-SPION, 200 µg/ml) as well as the longest heating duration (60 min). Thus, it is concluded that FA-PG-SPIONs with concentrations ≥ 100 µg/ml could be introduced and used as hyperthermia sensitizing agents leading to enhanced cancer therapy efficiency.

• Authors: Hamid Fakhimikabir, Mohamad Bagher Tavakoli, Ali Zarrabi, Alireza Amouheidari, Soheila Rahgozar
• Journal: Journal of thermal biology (J Therm Biol) Vol. 78 Pg. 73-83 (Dec 2018) ISSN: 0306-4565 [Print] England
• PMID: 30509670 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• Ferric Compounds
• Polymers
• ferric oxide
• polyglycerol
• Glycerol

Topics

• Ferric Compounds (chemistry)
• Glycerol (chemistry)
• HeLa Cells
• Humans
• Hyperthermia, Induced (methods)
• Metal Nanoparticles (adverse effects, chemistry, therapeutic use)
• Polymers (chemistry)