Dibenzoylmethane ameliorates lipid-induced inflammation and oxidative injury in diabetic nephropathy.

Abstract

Dibenzoylmethane (DBM) is a beta-diketone analog of curcumin. Numerous studies have shown the beneficial effects of curcumin on diabetes, obesity and diabetic complications including diabetic nephropathy. Recently, we investigated the beneficial metabolic effects of DBM on high-fat diet-induced obesity. However, the effects and mechanisms of action of DBM in the kidney are currently unknown. To investigate the renoprotective effects of DBM in type 2 diabetes, we administered DBM (100 mg/kg) orally for 12 weeks to high-fat diet-induced diabetic model mice. We used mouse renal mesangial (MES13) and macrophage (RAW 264.7) cells to examine the mechanism of action of DBM (20 μM). After DBM treatment, the albumin-to-creatinine ratio was significantly decreased compared to that of the high-fat-diet group. Moreover, damaged renal ultra-structures and functions including increased glomerular volume, glomerular basement membrane thickness and inflammatory signals were ameliorated after DBM treatment. Stimulation of MES13 and RAW264.7 cells by palmitate or high-dose glucose with lipopolysaccharides increased inflammatory signals and macrophage migration. However, these changes were reversed by DBM treatment. In addition, DBM inhibited NADPH oxidase 2 and 4 expression and oxidative DNA damage. Collectively, these data suggested that DBM prevented diabetes-induced renal injury through its anti-inflammatory and antioxidant effects.

• Authors: Eun Soo Lee, Mi-Hye Kwon, Hong Min Kim, Nami Kim, You Mi Kim, Hyeon Soo Kim, Eun Young Lee, Choon Hee Chung
• Journal: The Journal of endocrinology (J Endocrinol) Vol. 240 Issue 2 Pg. 169-179 (02 01 2019) ISSN: 1479-6805 [Electronic] England
• PMID: 30475214 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Chalcones
• Lipids
• dibenzoylmethane

Topics

• Animals
• Antioxidants (pharmacology)
• Cell Line
• Cell Movement (drug effects)
• Chalcones (pharmacology)
• Diabetes Mellitus, Type 2 (complications, etiology, prevention & control)
• Diabetic Nephropathies (etiology, prevention & control)
• Diet, High-Fat (adverse effects)
• Inflammation (chemically induced, prevention & control)
• Kidney (drug effects, pathology, ultrastructure)
• Kidney Glomerulus (drug effects, pathology, ultrastructure)
• Lipids
• Macrophages (cytology, drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• Oxidative Stress (drug effects)
• RAW 264.7 Cells