Probing carbohydrate metabolism using hyperpolarized 13 C-labeled molecules.

Abstract

Glycolysis is a fundamental metabolic process in all organisms. Anomalies in glucose metabolism are linked to various pathological conditions. In particular, elevated aerobic glycolysis is a characteristic feature of rapidly growing cells. Glycolysis and the closely related pentose phosphate pathway can be monitored in real time by hyperpolarized 13 C-labeled metabolic substrates such as 13 C-enriched, deuterated D-glucose derivatives, [2-13 C]-D-fructose, [2-13 C] dihydroxyacetone, [1-13 C]-D-glycerate, [1-13 C]-D-glucono-δ-lactone and [1-13 C] pyruvate in healthy and diseased tissues. Elevated glycolysis in tumors (the Warburg effect) was also successfully imaged using hyperpolarized [U-13 C6 , U-2 H7 ]-D-glucose, while the size of the preexisting lactate pool can be measured by 13 C MRS and/or MRI with hyperpolarized [1-13 C]pyruvate. This review summarizes the application of various hyperpolarized 13 C-labeled metabolites to the real-time monitoring of glycolysis and related metabolic processes in normal and diseased tissues.

• Authors: Jaspal Singh, Eul Hyun Suh, Gaurav Sharma, Chalermchai Khemtong, A Dean Sherry, Zoltan Kovacs
• Journal: NMR in biomedicine (NMR Biomed) Vol. 32 Issue 10 Pg. e4018 (10 2019) ISSN: 1099-1492 [Electronic] England
• PMID: 30474153 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Review)
• Copyright: © 2018 John Wiley & Sons, Ltd.

Chemical References

• Carbon Isotopes
• Carbon-13

Topics

• Animals
• Carbohydrate Metabolism
• Carbon Isotopes (metabolism)
• Glycolysis
• Humans
• Magnetic Resonance Spectroscopy
• Metabolome
• Time Factors