Preventive effect of Tongxinluo on endothelial survival and vascular integrity, together with inhibition of inflammatory reaction in rats model of intestine ischemia/reperfusion injury.

Abstract

This study was design to investigate preventive function of Tongxinluo (TXL) capsule on micro vascular function and endothelial survival in rats model of intestine ischemia/reperfusion (I/R) injury. We randomly divided fifty male Sprague-Dawley rats into Sham group, I/R group, TXL0.4+I/R group, TXL0.8+I/R group, TXL1.6+I/R group (10 rats each). Rat intestine I/R injury was carried out using a model of acute superior mesenteric artery occlusion with 30 min ischemia followed by 60 min reperfusion. The distribution of endothelial apoptosis in intestine was determined by CD31+TUNEL immunofluorescent double staining analysis. VE-Cadherin, ANGPTL4, HMGB1 and NF-κB were determined by immunohistochemical analysis. I/R induced massively endothelial cell apoptosis, accompanied with reduced expression of adherens junction protein VE-Cadherin and up regulation of inflammatory mediator HMGB1 and NF-κB. TXL pretreatment groups (TXL0.4+I/R, TXL0.8+I/R and TXL1.6+I/R group) significantly attenuated endothelial cell apoptosis with a dose-dependent effect. TXL pretreatment could maintain the expression of VE-Cadherin and promote the expression of ANGPTL4 which help to maintain endothelial integrity. TXL pretreatment also exert great influence in inhibiting HMGB1 expression and NF-κB expression induced by I/R. It could be concluded from this study that micro vascular dysfunction and endothelial damage play a causal role in rat intestine I/R injury. TXL pretreatment could significantly prevent the I/R induced pathology of endothelial apoptosis, micro vascular integrity disruption and inflammatory reaction.

• Authors: Jun-Xiu Zhang, Shao-Dan Li, Yi Liu, Ming-Hui Yang
• Journal: Pakistan journal of pharmaceutical sciences (Pak J Pharm Sci) Vol. 31 Issue 6 Pg. 2403-2410 (Nov 2018) ISSN: 1011-601X [Print] Pakistan
• PMID: 30473511 (Publication Type: Journal Article)

Chemical References

• ANGPTL4 protein, rat
• Angiopoietin-Like Protein 4
• Anti-Inflammatory Agents
• Antigens, CD
• Cadherins
• Drugs, Chinese Herbal
• Gastrointestinal Agents
• HMGB1 Protein
• Hbp1 protein, rat
• Inflammation Mediators
• NF-kappa B
• cadherin 5
• tongxinluo

Topics

• Angiopoietin-Like Protein 4 (metabolism)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antigens, CD (metabolism)
• Apoptosis (drug effects)
• Cadherins (metabolism)
• Capillary Permeability (drug effects)
• Cytoprotection
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Endothelial Cells (drug effects, metabolism, pathology)
• Gastrointestinal Agents (pharmacology)
• HMGB1 Protein (metabolism)
• Inflammation Mediators (metabolism)
• Intestines (blood supply)
• Male
• NF-kappa B (metabolism)
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)