ATRX promotes maintenance of herpes simplex virus heterochromatin during chromatin stress.

Abstract

The mechanisms by which mammalian cells recognize and epigenetically restrict viral DNA are not well defined. We used herpes simplex virus with bioorthogonally labeled genomes to detect host factors recruited to viral DNA shortly after its nuclear entry and found that the cellular IFI16, PML, and ATRX proteins colocalized with viral DNA by 15 min post infection. HSV-1 infection of ATRX-depleted fibroblasts resulted in elevated viral mRNA and accelerated viral DNA accumulation. Despite the early association of ATRX with vDNA, we found that initial viral heterochromatin formation is ATRX-independent. However, viral heterochromatin stability required ATRX from 4 to 8 hr post infection. Inhibition of transcription blocked viral chromatin loss in ATRX-knockout cells; thus, ATRX is uniquely required for heterochromatin maintenance during chromatin stress. These results argue that the initial formation and the subsequent maintenance of viral heterochromatin are separable mechanisms, a concept that likely extrapolates to host cell chromatin and viral latency.

• Authors: Joseph M Cabral, Hyung Suk Oh, David M Knipe
• Journal: eLife (Elife) Vol. 7 (11 22 2018) ISSN: 2050-084X [Electronic] England
• PMID: 30465651 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2018, Cabral et al.

Chemical References

• DNA, Viral
• Histones
• X-linked Nuclear Protein

Topics

• Cell Line
• Cell Line, Tumor
• DNA, Viral (genetics, metabolism)
• Fibroblasts (metabolism, virology)
• Gene Expression Regulation, Viral
• Gene Knockout Techniques
• HEK293 Cells
• Herpesvirus 1, Human (genetics, metabolism, physiology)
• Histones (metabolism)
• Host-Pathogen Interactions
• Humans
• Models, Genetic
• Virus Latency (genetics)
• X-linked Nuclear Protein (genetics, metabolism)