Abstract | BACKGROUND: MATERIAL AND METHODS: The study included 13 IBS patients according to Rome III criteria and 13 healthy donors. Freshly donated feces were administered to the descending part of the duodenum via a gastroscope. Feces were collected from donors and patients before FMT, and from the patients at 1, 3 and 12 weeks and donors and patients at 20/28 weeks after FMT. Microbiota analysis was performed using GA-map Dysbiosis test (Genetic Analysis AS, Oslo, Norway). The patients completed the following questionnaires before and at the aforementioned weeks after FMT: IBS Symptom Questionnaire (IBS-SQ), IBS-Symptom Severity Scoring system (IBS-SSS), Short Form of Nepean Dyspepsia Index (SF-NDI), Bristol stool form scale, the Eysenck Personality Questionnaire-Neuroticism and Hospital Anxiety and Depression. RESULTS: Donors and IBS patients had significantly different bacterial strain signals before FMT (Ruminococcus gnavus, Actinobacteria and Bifidobacteria) that became non-significant after 3 weeks following FMT. The changes in gut microbiota were similar between donors and patients at 20/28 weeks after FMT. Thus, patients' microbiota profiles became more-or-less similar to donors. The scores of all the questionnaires were significantly improved at all time points following FMT. No reported adverse effects. CONCLUSIONS: FMT was associated with a change in gut microbiota and improvement in IBS symptoms and quality of life lasting for up to 28 weeks. TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03333291.
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Authors | Tarek Mazzawi, Gülen Arslan Lied, Dag André Sangnes, Magdy El-Salhy, Johannes R Hov, Odd Helge Gilja, Jan Gunnar Hatlebakk, Trygve Hausken |
Journal | PloS one
(PLoS One)
Vol. 13
Issue 11
Pg. e0194904
( 2018)
ISSN: 1932-6203 [Electronic] United States |
PMID | 30427836
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adolescent
- Adult
- Aged
- Fecal Microbiota Transplantation
- Feces
(microbiology)
- Female
- Gastrointestinal Microbiome
(physiology)
- Humans
- Irritable Bowel Syndrome
(microbiology, therapy)
- Male
- Microbiota
- Middle Aged
- Quality of Life
- Young Adult
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