Overexpressed circ_0067934 acts as an oncogene to facilitate cervical cancer progression via the miR-545/EIF3C axis.

Abstract

Circular RNAs were recently identified as a novel type of noncoding RNAs. An increasing number of reports have demonstrated their essential regulatory roles in various biological processes and human diseases, including cancer. However, the role of circRNA in cervical cancer (CC) remains largely unknown. In the current study, we investigated the physiological functions of circ_0067934 during CC development and progression. We found that circ_0067934 was overexpressed in CC tissues and cell lines. Circ_0067934 upregulation was associated with advanced stage, lymph node metastasis, and poor prognosis in CC patients. Knockdown of circ_0067934 suppressed the proliferation, colony formation, migration, invasion, and epithelial-mesenchymal transition of CC cells in vitro. Circ_0067934 loss also inhibited CC tumor growth in vivo. Mechanistically, silencing circ_0067934 increased miR-545 expression. MiR-545 repressed EIF3C expression through targeting its 3'-untranslated region. MiR-545 suppressed the proliferation, migration, and invasion of CC cells, whereas restoration of EIF3C could rescue the effects of circ_0067934 knockdown. Taken together, our findings revealed that circ_0067934 promotes CC progression via miR-545/EIF3C axis. Our study may provide a new insight into the pathogenesis of CC.

• Authors: Chunjie Hu, Yue Wang, Ao Li, Jin Zhang, Fangfang Xue, Li Zhu
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 234 Issue 6 Pg. 9225-9232 (06 2019) ISSN: 1097-4652 [Electronic] United States
• PMID: 30362562 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Wiley Periodicals, Inc.

Chemical References

• EIF3C protein, human
• Eukaryotic Initiation Factor-3
• MIR545 microRNA, human
• MicroRNAs
• RNA, Circular

Topics

• Animals
• Base Sequence
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Epithelial-Mesenchymal Transition (genetics)
• Eukaryotic Initiation Factor-3 (genetics, metabolism)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Neoplasm Invasiveness
• Oncogenes
• RNA, Circular (genetics, metabolism)
• Up-Regulation (genetics)
• Uterine Cervical Neoplasms (genetics, pathology)