Abstract | AIM: Acinetobacter baumannii, an increasingly serious health threat, is considered as one of the six most dangerous microbes of high mortality rate. However, treatment of its infections is difficult because of the lack of efficient antibiotic or commercial vaccines. Passive immunization through administration of specific antibodies such as IgY, could be an attractive practical solution. METHODS AND RESULTS: In the current study, antigenicity of two recombinant outer membrane proteins (OmpA and Omp34) as well as inactivated whole cell of A. baumannii was assessed by ELISA. Moreover, prophylactic effects of specific IgY antibodies (avian antibody) raised against these antigens were evaluated in a murine pneumonia model. The specific IgY antibodies had various prophylactic effects in the pneumonia model. OmpA was the most potent antigen in terms of triggering antibody and conferring protection. While a synergic effect was observed in ELISA for antibodies raised against a combination of OmpA and Omp34 (which are known as Omp33-36 and Omp34 kDa), an antagonistic effect was unexpectedly seen in challenges. The reason for this phenomenon remains to be precisely addressed. CONCLUSION: SIGNIFICANCE AND IMPACT OF THE STUDY:
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Authors | A Jahangiri, P Owlia, I Rasooli, J Salimian, E Derakhshanifar, A Naghipour Erami, E Darzi Eslam, S Darvish Alipour Astaneh |
Journal | Journal of applied microbiology
(J Appl Microbiol)
Vol. 126
Issue 2
Pg. 624-632
(Feb 2019)
ISSN: 1365-2672 [Electronic] England |
PMID | 30353977
(Publication Type: Journal Article)
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Copyright | © 2018 The Society for Applied Microbiology. |
Chemical References |
- Antigens, Bacterial
- Bacterial Outer Membrane Proteins
- IgY
- Immunoglobulins
- OMPA outer membrane proteins
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Topics |
- Acinetobacter Infections
(prevention & control)
- Acinetobacter baumannii
(immunology)
- Animals
- Antigens, Bacterial
(immunology)
- Bacterial Outer Membrane Proteins
(immunology)
- Egg Yolk
(immunology)
- Immunization, Passive
- Immunoglobulins
(immunology)
- Mice
- Pneumonia, Bacterial
(prevention & control)
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