Effect of anakinra, an interleukin one beta antagonist, on oxidative testicular damage induced in rats with ischemia reperfusion.

Abstract

BACKGROUND:

It has been reported in the literature that proinflammatory interleukin-1 beta (IL-1β) is increased in cases of testicular ischemia reperfusion (I/R) damage. This information suggests that anakinra, an IL-1β antagonist, may be effective in testicular I/R damage.

OBJECTIVE:

In our study, we investigated the effect of anakinra on testicular I/R damage induced in rats with torsion/detorsion.

METHODS:

The 50mg/kg anakinra+testicular torsion/detorsion (KTD-50) and 100mg/kg anakinra+testicular torsion/detorsion (KTD-100) groups received an intraperitoneal (i.p.) injection of 50mg/kg and 100mg/kg of anakinra, respectively. In turn, the testicular torsion/detorsion (TTD) and sham operation (SOG) groups received a single dose of distilled water as a solvent 1h before ketamine anaesthesia. After the testes of the TTD, KTD-50 and KTD-100 groups were subjected to torsion and detorsion for 4h each, the rats were killed with a high-dose anaesthesia, and their testicles were removed and evaluated through biochemical, gene expression and histopathological examinations. The results were evaluated in comparison with those of the SOG group.

RESULTS:

The levels of malondialdehyde (MDA), myeloperoxidase (MPO) and IL-1β showed significant increases in the TTD group, which underwent torsion/detorsion, compared to the KTD-50, KTD-100 and SOG groups. Conversely, the levels of glutathione (tGSH), glutathione peroxidase (GPO) and glutathione s-transferase (GST) were found to be significantly higher in the KTD-50, KTD-100 and SOG groups than in the TTD group.

CONCLUSION:

Anakinra at a 100mg/kg dose histologically suppressed better oxidative stress and tunica albuginea, germ cell, seminiferous tubule and interstitial damage in the testicular tissue compared to a 50mg/kg dose. Experimental results indicate that anakinra might be beneficial in the attenuation of testicular I/R damage.

• Authors: Erkan Hirik, Bahadir Suleyman, Renad Mammadov, Turgut Yapanoglu, Ferda Keskin Cimen, Nihal Cetin, Nezahat Kurt
• Journal: Revista internacional de andrologia (Rev Int Androl) 2018 Jul - Sep Vol. 16 Issue 3 Pg. 87-94 ISSN: 1698-0409 [Electronic] Spain
• PMID: 30300135 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2017 Asociación Española de Andrología, Medicina Sexual y Reproductiva. Publicado por Elsevier España, S.L.U. All rights reserved.

Chemical References

• Interleukin 1 Receptor Antagonist Protein
• Interleukin-1beta
• Malondialdehyde
• Peroxidase
• Glutathione Peroxidase
• Glutathione

Topics

• Animals
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Interleukin 1 Receptor Antagonist Protein (administration & dosage, pharmacology)
• Interleukin-1beta (antagonists & inhibitors, metabolism)
• Male
• Malondialdehyde (metabolism)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy)
• Seminiferous Tubules (drug effects, pathology)
• Spermatic Cord Torsion (drug therapy)