A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model.

Abstract

Peroxynitrite (ONOO-) plays important roles in the regulation of many physiological and pathological processes, and an increase in its levels is related to numerous diseases. Thus, accurate detection of ONOO- in physiological conditions is imperative for elucidating its functions. However, studies on high signal-to-noise-ratio (SNR) fluorescence imaging of ONOO-in vivo for its detection are currently lacking. Thus, a novel NIR xanthene fluorescence probe (NOF2) for the endogenous detection of ONOO- is designed and synthesized. The fluorescence of the NOF2 probe is pre-quenched by the hydroxyl protection group of diphenyl phosphinate. Additionally, the NOF2 probe exhibits good selectivity and sensitivity for ONOO- with a low detection limit of 0.40 μM. Importantly, the NOF2 probe displays good performances for the detection of endogenous ONOO- not only in living cells but also in a mouse inflammation model. This demonstrates its great potential for applications involving the detection of ONOO- both in vitro and in vivo to explore the roles of ONOO- in different physiological systems.

• Authors: Yongquan Wu , Aiping Shi , Yuanyan Li , Hong Zeng , Xiaoyong Chen , Jie Wu , Xiaolin Fan
• Journal: The Analyst (Analyst) Vol. 143 Issue 22 Pg. 5512-5519 (Nov 05 2018) ISSN: 1364-5528 [Electronic] England
• PMID: 30295297 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Phosphinic Acids
• Xanthenes
• Peroxynitrous Acid

Topics

• Animals
• Fluorescence
• Fluorescent Dyes (chemical synthesis, chemistry, toxicity)
• Humans
• Inflammation (metabolism)
• Limit of Detection
• MCF-7 Cells
• Mice
• Peroxynitrous Acid (analysis)
• Phosphinic Acids (chemical synthesis, chemistry, toxicity)
• RAW 264.7 Cells
• Spectrometry, Fluorescence (methods)
• Xanthenes (chemical synthesis, chemistry, toxicity)