Subchronic toxicity evaluation of 5-hexenyl isothiocyanate, a nature identical flavoring substance from Wasabia japonica, in F344/DuCrj rats.

Abstract

5-Hexenyl isothiocyanate (5-HeITC) is a naturally derived flavoring substance from Wasabia japonica. To clarify the toxicological profile of 5-HeITC, we performed a subchronic toxicity study of 5-HeITC with intragastric administration at daily doses of 0, 3, 12, or 48 mg/kg body weight (BW) to 6-week-old male and female F344/DuCrj rats for 13 weeks. Body weight gain was decreased in the male 48 mg/kg BW group. Decreased triglycerides were observed in the male over 12 mg/kg BW and female 48 mg/kg BW groups. Decreased total cholesterol was observed in the male 48 mg/kg BW group. Increases in relative liver weights were observed in the male 48 mg/kg BW and female over 12 mg/kg BW groups. Increases in absolute and relative heart weights were observed in the female over 12 mg/kg BW groups. Simple hyperplasia in the urinary bladder was found in the male and female 12 mg/kg BW groups, and nodular hyperplasia was found in the female 48 mg/kg BW group. Based on these findings, the target organs of 5-HeITC were determined to be the urinary bladder, heart, and liver. The no-observed-adverse-effect level of 5-HeITC for both sexes was estimated to be 3 mg/kg BW.

• Authors: Jun-Ichi Akagi, Young-Man Cho, Yasuko Mizuta, Takeshi Toyoda, Kumiko Ogawa
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 122 Pg. 80-86 (Dec 2018) ISSN: 1873-6351 [Electronic] England
• PMID: 30266315 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Ltd.

Chemical References

• Flavoring Agents
• Isothiocyanates
• 5-hexenyl isothiocyanate

Topics

• Administration, Oral
• Animals
• Body Weight (drug effects)
• Clinical Chemistry Tests
• Dose-Response Relationship, Drug
• Female
• Flavoring Agents (administration & dosage, toxicity)
• Heart (drug effects)
• Hematologic Tests
• Hyperplasia (chemically induced)
• Isothiocyanates (administration & dosage, toxicity)
• Liver (drug effects)
• Male
• No-Observed-Adverse-Effect Level
• Organ Size (drug effects)
• Rats, Inbred F344
• Toxicity Tests, Subchronic
• Urinary Bladder (pathology)
• Wasabia (chemistry)