Protective Effect of Baicalin Against TLR4-mediated UVA-induced Skin Inflammation.

Abstract

UVA irradiation is known to cause photoaging via production of reactive oxygen species (ROS) and activation of inflammatory processes. Previously, we have demonstrated that baicalin, a plant-derived flavonoid possessing both antioxidant and anti-inflammatory activity, protects mouse keratinocytes against damage from UVB irradiation. However, the role of baicalin in vivo has not been well studied, particularly in the setting of UVA irradiation. To explore the protective effects and mechanisms of baicalin treatment in mice after UVA irradiation, mice were exposed to acute and chronic doses of UVA irradiation with or without baicalin or vehicle. Skin samples were collected for histological staining, RNA isolation, flow cytometry and protein extraction. Our results demonstrate the protective effect of baicalin against UVA-induced oxidative damage and inflammation in mouse skin. These effects are likely mediated via the TLR4 pathway, which may serve as a target for photochemoprevention against skin inflammation.

• Authors: Mohammad Asif Sherwani, Kevin Yang, Aditi Jani, Reham A Abed, Abdul Karim Taufique, Tolulope Gid Dosunmu, Nabiha Yusuf
• Journal: Photochemistry and photobiology (Photochem Photobiol) Vol. 95 Issue 2 Pg. 605-611 (03 2019) ISSN: 1751-1097 [Electronic] United States
• PMID: 30246296 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The American Society of Photobiology.

Chemical References

• Flavonoids
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• baicalin

Topics

• Animals
• Dermatitis (etiology, prevention & control)
• Female
• Flavonoids (pharmacology)
• Mice
• Mice, Inbred C3H
• Skin (physiopathology, radiation effects)
• Toll-Like Receptor 4 (physiology)
• Ultraviolet Rays