Antimicrobial Effect of Peptide DJK-5 Used Alone or Mixed with EDTA on Mono- and Multispecies Biofilms in Dentin Canals.

Abstract

INTRODUCTION:

The present study aimed to evaluate the antibacterial effect of a new peptide, DJK-5, used alone or mixed together with EDTA on mono- and multispecies biofilms in dentin canals covered by a smear layer with or without preceding sodium hypochlorite (NaOCl) irrigation.

METHODS:

One hundred twelve dentin blocks (224 final specimens) were prepared and divided into 56 groups, and Enterococcus faecalis or multispecies bacteria were introduced into dentinal tubules by centrifugation. After 1 week of cultivation, a uniform smear layer was created on the surface of the dentin blocks, and the samples were exposed to sterile water, 17% EDTA, 2% or 6% NaOCl, 10 μg/mL DJK-5, or a mixture of 8.5% EDTA +10 μg/mL DJK-5 or were combined treated with the solution in the following sequence: 2% or 6% NaOCl +10 μg/mL DJK-5, 2% or 6% NaOCl + 8.5% EDTA +10 μg/mL DJK-5, 2% or 6% NaOCl + 8.5% EDTA + 10 μg/mL DJK-5. Specimens without a smear layer treated by 6% NaOCl or 10 μg/mL DJK-5 served as the positive control. The irrigant exposure time was 3 or 10 minutes. The antibacterial efficacy was determined by live/dead staining and confocal laser scanning microscopy.

RESULTS:

The smear layer reduced the antibacterial capacity of 6% NaOCl and 10 μg/mL DJK-5. The efficacy of 2% or 6% NaOCl followed by 10 μg/mL DJK-5 was superior to 10 μg/mL DJK-5 alone (P < .05) but inferior to 2% or 6% NaOCl + 8.5% EDTA + 10 μg/mL DJK-5 and 2% or 6% NaOCl + 8.5% EDTA + 10 μg/mL DJK-5 (P < .05). The mixture of 8.5% EDTA and 10 μg/mL DJK-5 had the same disinfection effectiveness as 10 μg/mL DJK-5 used alone (P < .05). Using 2% or 6% NaOCl before EDTA + peptide always resulted in the highest killing (P < .05).

CONCLUSIONS:

The smear layer inhibits the disinfectant effect in dentin. Peptide DJK-5 showed a strong antibacterial effect against mono- and multispecies biofilms in dentin canals. The highest killing was measured when 6% NaOCl was followed by a mixture of EDTA and peptide DJK-5.

• Authors: Dan Wang, Ya Shen, Robert E W Hancock, Jingzhi Ma, Markus Haapasalo
• Journal: Journal of endodontics (J Endod) Vol. 44 Issue 11 Pg. 1709-1713 (Nov 2018) ISSN: 1878-3554 [Electronic] United States
• PMID: 30243660 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• DJK-5 peptide
• Drug Combinations
• Oligopeptides
• Root Canal Irrigants
• Edetic Acid
• Sodium Hypochlorite

Topics

• Anti-Bacterial Agents
• Biofilms (drug effects)
• Dental Pulp Cavity (microbiology)
• Dose-Response Relationship, Drug
• Drug Combinations
• Drug Resistance, Bacterial
• Edetic Acid (administration & dosage, pharmacology)
• Enterococcus faecalis (drug effects)
• Humans
• In Vitro Techniques
• Oligopeptides (administration & dosage, pharmacology)
• Root Canal Irrigants (administration & dosage)
• Smear Layer (microbiology)
• Sodium Hypochlorite (administration & dosage)