The combined effect of granulocyte-colony stimulating factor (G-CSF) treatment and exercise in rats with spinal cord injury.

Abstract

Objective: To identify that the combined G-CSF and treadmill exercise is more effective in functional recovery after spinal cord injury (SCI).Design: Rats were divided into 4 groups: a SCI group treated with G-CSF (G-CSF group, n = 6), a SCI group treated with treadmill exercise plus G-CSF (G-CSF/exercise group, n = 6), a SCI group with treadmill exercise (exercise group, n = 6), and a SCI group without treatments (control group, n = 6). We performed laminectomy at the T8-10 spinal levels with compression injury of the spinal cord in all rats. G-CSF (20 μg/ml) was administered intraperitoneally for 5 consecutive days after SCI in G-CSF and G-CSF/exercise groups. From one week after surgery, animals in G-CSF/exercise and exercise groups received 30 min of exercise 5 days per week for 4 weeks. Functional recoveries were assessed using the Basso, Beattie, and Bresnahan (BBB) scale and the inclined plane test. Five weeks after SCI, hematoxylin and eosin staining for cavity size and immunohistochemistry for glial scar formation and neuro-regeneration factor expression were conducted.Setting: Inha University School of medicine, Incheon, KoreaResults: Rats in G-CSF/exercise group showed the most effective functional recovery in the BBB scale and the inclined plane test, and spinal cord cavity size by injury were the smallest, and immunohistochemistry revealed expression of higher BDNF (brain-derived neurotrophic factor) and VEGF (vascular endothelial growth factor) and lower GFAP (glial fibrillary acidic protein) than others.Conclusion: Combined treatment provided more effective neuroplasty and functional recovery than individual treatments.

• Authors: Chan-Hyuk Park, Kyung-Lim Joa, Mi-Ok Lee, Seung-Hwan Yoon, Myeong-Ok Kim
• Journal: The journal of spinal cord medicine (J Spinal Cord Med) Vol. 43 Issue 3 Pg. 339-346 (05 2020) ISSN: 2045-7723 [Electronic] England
• PMID: 30230978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bdnf protein, rat
• Brain-Derived Neurotrophic Factor
• GFAP protein, rat
• Glial Fibrillary Acidic Protein
• Vascular Endothelial Growth Factor A
• vascular endothelial growth factor A, rat
• Granulocyte Colony-Stimulating Factor

Topics

• Animals
• Behavior, Animal (physiology)
• Brain-Derived Neurotrophic Factor (metabolism)
• Combined Modality Therapy
• Disease Models, Animal
• Exercise Test
• Exercise Therapy
• Glial Fibrillary Acidic Protein (metabolism)
• Granulocyte Colony-Stimulating Factor (administration & dosage, pharmacology)
• Infusions, Parenteral
• Male
• Physical Conditioning, Animal (physiology)
• Rats
• Rats, Sprague-Dawley
• Recovery of Function (drug effects, physiology)
• Spinal Cord Injuries (drug therapy, metabolism, pathology, therapy)
• Vascular Endothelial Growth Factor A (metabolism)