Translocation-generated ITK-FER and ITK-SYK fusions induce STAT3 phosphorylation and CD69 expression.

Abstract

Many cancer types carry mutations in protein tyrosine kinase (PTK) and such alterations frequently drive tumor progression. One category is gene translocation of PTKs yielding chimeric proteins with transforming capacity. In this study, we characterized the role of ITK-FER [Interleukin-2-inducible T-cell Kinase (ITK) gene fused with Feline Encephalitis Virus-Related kinase (FER) gene] and ITK-SYK [Interleukin-2-inducible T-cell Kinase (ITK) gene fused with the Spleen Tyrosine Kinase (SYK)] in Peripheral T Cell Lymphoma (PTCL) signaling. We observed an induction of tyrosine phosphorylation events in the presence of both ITK-FER and ITK-SYK. The downstream targets of ITK-FER and ITK-SYK were explored and STAT3 was found to be highly phosphorylated by these fusion kinases. In addition, the CD69 T-cell activation marker was significantly elevated. Apart from tyrosine kinase inhibitors acting directly on the fusions, we believe that drugs acting on downstream targets could serve as alternative cancer therapies for fusion PTKs.

• Authors: Narmeen N Fathi, Dara K Mohammad, André Görgens, Samir El Andaloussi, Rula Zain, Beston F Nore, C I Edvard Smith
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 504 Issue 4 Pg. 749-752 (10 12 2018) ISSN: 1090-2104 [Electronic] United States
• PMID: 30217447 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Antigens, CD
• Antigens, Differentiation, T-Lymphocyte
• CD69 antigen
• Lectins, C-Type
• Oncogene Proteins, Fusion
• STAT3 Transcription Factor
• proto-oncogene protein c-fes-fps
• Protein-Tyrosine Kinases
• SYK protein, human
• Syk Kinase
• emt protein-tyrosine kinase

Topics

• Animals
• Antigens, CD (metabolism)
• Antigens, Differentiation, T-Lymphocyte (metabolism)
• COS Cells
• Chlorocebus aethiops
• HEK293 Cells
• Humans
• Jurkat Cells
• Lectins, C-Type (metabolism)
• Lymphoma, T-Cell, Peripheral (genetics, metabolism, pathology)
• Mice
• NIH 3T3 Cells
• Oncogene Proteins, Fusion (genetics, metabolism)
• Phosphorylation
• Protein-Tyrosine Kinases (genetics, metabolism)
• STAT3 Transcription Factor (metabolism)
• Syk Kinase (genetics, metabolism)
• Translocation, Genetic