[Determination of flavonoids constituents in epimedium total flavone capsule and research on its anti-osteoporosis activity].

Abstract

To develop a rapid resolution liquid chromatography (RRLC) method for the simultaneous determination of epimedoside A, epimedin A1, epimedin A, epimedin B, epimedin C, icariin, baohuosideⅡ, icarisideⅠ, sagittatoside B, 2"-O-rhamnosyl icarisideⅡ, and baohuosideⅠin epimedium total flavone capsule. At the same time, the effects of the above 11 compounds on osteogenic differentiation of MC3T3-E1 cells were investigated by detecting the content of alkaline phosphatase (AKP). The results showed that baohuoside Ⅱ had the highest activities, and both the activities of baohuoside Ⅱ and icariside Ⅰ were stronger than those of icariin.In this study, the content determination method of flavonoid glycosides was established, and the anti-osteoporosis effect of monomers was compared, providing technical support for the study of the pharmacodynamic and mechanism of Epimedium total flavone capsule.

• Authors: Zhong-Kun Xu, Hong-Mei Yin, Fang Li, Jian-Ping Qin, Shao-Li Gu, Yun Wu, Zhen-Zhong Wang, Wei Xiao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 43 Issue 15 Pg. 3140-3144 (Aug 2018) ISSN: 1001-5302 [Print] China
• PMID: 30200709 (Publication Type: Journal Article)
• Copyright: Copyright© by the Chinese Pharmaceutical Association.

Chemical References

• Flavones
• Flavonoids
• Phytochemicals

Topics

• 3T3 Cells
• Animals
• Epimedium (chemistry)
• Flavones (analysis)
• Flavonoids (analysis)
• Mice
• Osteogenesis (drug effects)
• Osteoporosis
• Phytochemicals (analysis)