Abstract |
Glioblastomas (GBMs) are highly aggressive brain tumors with a dismal prognosis. Nuclear factor I (NFI) is a family of transcription factors that controls glial cell differentiation in the developing central nervous system. NFIs have previously been shown to regulate the expression of astrocyte markers such as glial fibrillary acidic protein (GFAP) in both normal brain and GBM cells. We used chromatin immunoprecipitation (ChIP)-on-chip to identify additional NFI targets in GBM cells. Analysis of our ChIP data revealed ~400 putative NFI target genes including an effector of the Notch signaling pathway, HEY1, implicated in the maintenance of neural stem cells. All four NFIs (NFIA, NFIB, NFIC, and NFIX) bind to NFI recognition sites located within 1 kb upstream of the HEY1 transcription site. We further showed that NFI negatively regulates HEY1 expression, with knockdown of all four NFIs in GBM cells resulting in increased HEY1 RNA levels. HEY1 knockdown in GBM cells decreased cell proliferation, increased cell migration, and decreased neurosphere formation. Finally, we found a general correlation between elevated levels of HEY1 and expression of the brain neural stem/progenitor cell marker B-FABP in GBM cell lines. Knockdown of HEY1 resulted in an increase in the RNA levels of the GFAP astrocyte differentiation marker. Overall, our data indicate that HEY1 is negatively regulated by NFI family members and is associated with increased proliferation, decreased migration, and increased stem cell properties in GBM cells.
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Authors | Miranda Brun, Saket Jain, Elizabeth A Monckton, Roseline Godbout |
Journal | Neoplasia (New York, N.Y.)
(Neoplasia)
Vol. 20
Issue 10
Pg. 1023-1037
(10 2018)
ISSN: 1476-5586 [Electronic] United States |
PMID | 30195713
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Basic Helix-Loop-Helix Transcription Factors
- Cell Cycle Proteins
- HEY1 protein, human
- NFI Transcription Factors
- Repressor Proteins
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Topics |
- Basic Helix-Loop-Helix Transcription Factors
(genetics, metabolism)
- Binding Sites
- Cell Cycle Proteins
(genetics, metabolism)
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Chromatin Immunoprecipitation
(methods)
- Gene Expression Regulation, Neoplastic
- Glioblastoma
(genetics, pathology)
- Humans
- NFI Transcription Factors
(genetics, metabolism)
- Neoplastic Stem Cells
(pathology)
- Promoter Regions, Genetic
- Repressor Proteins
(genetics, metabolism)
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