Accuracy of heparin binding protein: as a new marker in prediction of acute bacterial meningitis.

Abstract

BACKGROUND:

Cerebrospinal fluid bacterial culture is the gold-standard for confirmation of acute bacterial meningitis, but many cases are not culture confirmed. Antibiotics reduce the chance of a microbiological diagnosis. Objective to evaluate efficacy of Heparin-binding protein in diagnosis of bacterial meningitis.

PATIENTS:

30 patients diagnosed with acute bacterial meningitis, 30 viral meningitis, and 30 subjects with normal CSF findings.

DESIGN:

Diagnosis was based on history, clinical criteria, CSF examination, latex agglutination & culture, and sensitivities and response to therapy. HBP was measured using enzyme-linked immunosorbent technique in both serum & CSF.

RESULTS:

Cerebrospinal fluid HBP levels averaged 0.82±0.3ng/mL in controls, 3.3±1.7ng/mL in viral and 174.8±46.7ng/mL in bacterial meningitis. Mean serum level was 0.84±0.3ng/mL in the controls, 3.7±1.9ng/mL in viral, and 192.2±56.6ng/mL in bacterial meningitis. Both HBP levels were significantly higher in patients with bacterial meningitis. Cut-offs of 56.7ng/ml and 45.3ng/ml in cerebrospinal fluid & serum showed 100% overall accuracy. Even in patients who received prior antibiotics, remained elevated.

CONCLUSION:

Serum Heparin-binding protein serves as a non-invasive potential marker of acute bacterial meningitis even in partially treated cases.

• Authors: Mona Kandil, Gihane Khalil, Eman El-Attar, Gihan Shehata, Salwa Hassan
• Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (Braz J Microbiol) Vol. 49 Suppl 1 Pg. 213-219 (Nov 2018) ISSN: 1678-4405 [Electronic] Brazil
• PMID: 30166267 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

Chemical References

• AZU1 protein, human
• Antimicrobial Cationic Peptides
• Biomarkers
• Blood Proteins
• Carrier Proteins
• Heparin

Topics

• Adolescent
• Adult
• Antimicrobial Cationic Peptides (blood, cerebrospinal fluid)
• Biomarkers (blood, cerebrospinal fluid)
• Blood Proteins (cerebrospinal fluid)
• Carrier Proteins (blood, cerebrospinal fluid)
• Child
• Child, Preschool
• Cross-Sectional Studies
• Female
• Heparin (metabolism)
• Humans
• Infant
• Male
• Meningitis, Bacterial (blood, cerebrospinal fluid, diagnosis, microbiology)
• Middle Aged
• Young Adult