Development and evaluation of in murine model, of an improved live-vaccine candidate against brucellosis from to Brucella melitensis vjbR deletion mutant.

Abstract

Brucellosis is an infectious disease that brings enormous economic burdens for developing countries. The Brucella melitensis (B. melitensis) M5-90 vaccine strain (M5-90) has been used on a large scale in China, but may cause abortions if given to pregnant goats or sheep subcutaneously during the late stages of gestation. Moreover, the vaccine M5-90 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent M5-90 vaccine is required. In this study, a vjbR mutant of M5-90 (M5-90ΔvjbR) was constructed and overcame these drawbacks. M5-90ΔvjbR strain showed reduced survival capability in murine macrophages (RAW 264.7) and BALB/c mice and induced high protective immunity in mice. In addition, M5-90ΔvjbR induced an anti-Brucella-specific immunoglobulin G (IgG) response and stimulated the expression of gamma interferon (INF-γ) and interleukin-4 (IL-4) in vaccinated mice. Furthermore, M5-90ΔvjbR induced IgG response and stimulated the secretion of IFN-γ and IL-4 in immunized sheep. Moreover, the VjbR antigen allowed serological differentiation between infected and vaccinated animals. These results suggest that M5-90ΔvjbR is an ideal live attenuated and efficacious live vaccine candidate against B. melitensis 16 M infection.

• Authors: Zhiqiang Li, Shuli Wang, Hui Zhang, Li Xi, Jinliang Zhang, Xiaogen Zhang, Qingfeng Zhou, Jihai Yi, Min Li, Weihua Zhang, Junbo Zhang
• Journal: Microbial pathogenesis (Microb Pathog) Vol. 124 Pg. 250-257 (Nov 2018) ISSN: 1096-1208 [Electronic] England
• PMID: 30149131 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Ltd.

Chemical References

• Antibodies, Bacterial
• Bacterial Proteins
• Brucella Vaccine
• Vaccines, Attenuated
• Interleukin-4
• Interferon-gamma

Topics

• Animals
• Antibodies, Bacterial (immunology)
• Bacterial Proteins (administration & dosage, genetics, immunology)
• Brucella Vaccine (administration & dosage, genetics, immunology)
• Brucella melitensis (genetics, immunology)
• Brucellosis (immunology, microbiology, prevention & control)
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Female
• Gene Deletion
• Humans
• Interferon-gamma (genetics, immunology)
• Interleukin-4 (genetics, immunology)
• Mice
• Mice, Inbred BALB C
• Sequence Deletion
• Vaccines, Attenuated (administration & dosage, genetics, immunology)