Deep sequencing profiles MicroRNAs related to Aspergillus fumigatus infection in lung tissues of mice.

Abstract

BACKGROUND:

Invasive pulmonary aspergillosis (IPA) is a severe opportunistic infection with high mortality in patients with compromised immunity. The full repertoire of microRNAs (miRNAs) involved in the regulation of IPA infection remains to be established.

METHODS:

We established a mouse IPA model and analyzed small RNA transcriptomes in lung tissues of immunodeficient IPA mice (IPA group) and matched immunodeficient control mice (control group) through next-generation sequencing.

RESULTS:

A total of 3759 known miRNAs were detected, in which 23 miRNAs were identified to be related to IPA. IPA-associated miRNAs include upregulated mmu-let-7b-3p, mmu-miR124-3p, mmu-miR21a-3p, mmu-miR29c-5p, mmu-miR3473b and mmu-miR3473e, and downregulated mmu-miR-150-3p and mmu-miR-503-5p. The expression levels of eight identified miRNAs were quantified in a validation cohort (n = 40) by qRT-PCR, and results revealed the same change patterns. MiRNA target prediction revealed that all IPA-related miRNAs possibly engage a cooperative regulation of key elements in the NF-kappa B signaling pathway.

CONCLUSION:

We conclude that deep-sequencing small RNAs can uncover miRNA pool-regulating IPA. Our results may lead to further understanding IPA pathogenesis and gain insight into the complexity and diversity of small RNA molecules that regulate immunodeficient IPA.

• Authors: Fei Chen, Xiao-Yong Xu, Ming Zhang, Chen Chen, Hong-Tao Shao, Yi Shi
• Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (J Microbiol Immunol Infect) Vol. 52 Issue 1 Pg. 90-99 (Feb 2019) ISSN: 1995-9133 [Electronic] England
• PMID: 30146414 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier B.V.

Chemical References

• MicroRNAs

Topics

• Animals
• Aspergillus fumigatus
• Disease Models, Animal
• Female
• Gene Expression Profiling
• Gene Expression Regulation
• High-Throughput Nucleotide Sequencing
• Invasive Pulmonary Aspergillosis (genetics, microbiology, pathology)
• Lung (microbiology, pathology)
• Mice, Inbred C57BL
• MicroRNAs (genetics)
• Reproducibility of Results
• Sequence Analysis, RNA
• Signal Transduction (genetics)