Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium.

Abstract

OBJECTIVE:

To directionally-differentiate dermis-derived mesenchymal stem cells (DMSCs) into vascular endothelial cells (VECs) in vitro, providing an experimental basis for studies on the pathogenesis and treatment of vascular diseases.

METHODS:

After separation by adherent culture, VEC line supernatant, vascular endothelial growth factor (VEGF), bone morphogenetic protein-4 and hypoxia were used for the differentiation of VECs from DMSCs. The cell type was authenticated by flow cytometry, matrigel angiogenesis assay in vitro, and immunofluorescent staining during differentiation. The VEGF concentration was investigated by enzyme-linked immunosorbent assay.

RESULTS:

After 28 days of differentiation, the cell surface marker CD31 was significantly positive (80%-90%) by flow cytometry in the VEC line-conditioned culture, which was significantly higher than in the other groups. Differentiated DMSCs had the ability to ingest Dil-ac-LDL and vascularize in the conditioned culture, but not in the other groups. In the VEC line supernatant, the concentration of VEGF was very low. The VEGF concentration changed along with the differentiation into VECs in the medium of the conditioned culture group.

CONCLUSION:

VEC line supernatant can induce the differentiation of DMSCs into VECs, possibly through the pathway except VEGF.

• Authors: Ling Zhou, Xuping Niu, Jiannan Liang, Junqin Li, Jiao Li, Yueai Cheng, Yanfeng Meng, Qiang Wang, Xiaoli Yang, Gang Wang, Yu Shi, Erle Dang, Kaiming Zhang
• Journal: Acta histochemica (Acta Histochem) Vol. 120 Issue 8 Pg. 734-740 (Nov 2018) ISSN: 1618-0372 [Electronic] Germany
• PMID: 30143315 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier GmbH. All rights reserved.

Chemical References

• Culture Media, Conditioned
• Platelet Endothelial Cell Adhesion Molecule-1
• Vascular Endothelial Growth Factor A

Topics

• Adult
• Cell Culture Techniques
• Cell Differentiation
• Culture Media, Conditioned
• Dermis (cytology)
• Endothelial Cells (cytology)
• Endothelium (cytology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Flow Cytometry
• Humans
• Male
• Mesenchymal Stem Cells (cytology)
• Platelet Endothelial Cell Adhesion Molecule-1 (metabolism)
• Vascular Endothelial Growth Factor A